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Data of The Expression of Serotonin in Alzheimer's Disease (AD) Rat Model Under Treatment of Ethanolic Extract Ocimum sanctum Linn.

ABSTRACT: The article offers dataset on the expression of serotonergic nerve in the hippocampal area of Alzheimer's disease (AD) model. Since decreasing expression of serotonin linked to dementia, this data will help the neuroscientist, who work on neurodegeneration. This dataset demonstrates the potential of Ocimum sanctum Extract (OSE) as a neuroprotective and neurodegenerative agent against AD. The OSE mechanism focusing on the expression of serotonin as a therapeutic target. To acquire the dataset, we approached using two models, in vitro and in vivo. On the In vivo model, used two months old 27 male rats and divided into three groups, non-treated (Group A, n=9), AD rats model pre-treated with OSE followed induction for TMT on the days of seventh (group B, n=9) and AD rats model treated with OSE both on pre-TMT introduction for seven days and post-TMT induction for 21 days (group C, n=9). AD rats euthanised on day seventh; 14th; and 21st. The brain samples were analysed for neuronal density and serotonin immunoreactivity qualitatively. Besides, In Vitro's data were collected from HEK-293 cells which induce by TMT as of AD model. The data expression of serotonin on the in-vitro model analysed using ELISA method.

SUBMITTER: Raditya MN

PROVIDER: S-EPMC7206209 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature

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Data of The Expression of Serotonin in Alzheimer's Disease (AD) Rat Model Under Treatment of Ethanolic Extract Ocimum sanctum Linn.

Raditya M Nova MN Bagus A M Made AMM Kustiati Ulayatul U Wihadmadyatami Hevi H Kusindarta Dwi Liliek DL

Data in brief 20200430

The article offers dataset on the expression of serotonergic nerve in the hippocampal area of Alzheimer's disease (AD) model. Since decreasing expression of serotonin linked to dementia, this data will help the neuroscientist, who work on neurodegeneration. This dataset demonstrates the potential of <i>Ocimum sanctum</i> Extract (OSE) as a neuroprotective and neurodegenerative agent against AD. The OSE mechanism focusing on the expression of serotonin as a therapeutic target. To acquire the data ...[more]

PMID: 32395598

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Project description:Background and aimLung cancer, especially non-small cell lung cancer (NSCLC), has been identified as the leading cause of cancer deaths worldwide. The mortality rate from lung cancer has been estimated to be 18.4%. Until now, conventional treatments have not yielded optimal results, thus necessitating an investigation into the use of traditional herbal plants as potential candidates for its treatment. This study aimed to determine the inhibitory and apoptotic activity of the ethanolic extract from Ocimum sanctum Linn. (EEOS) by in silico molecular docking and through in vitro studies using NSCLC cells (A549 cell line).Materials and methodsDried simplicia of Ocimum sanctum was converted into EEOS using the maceration method. Spectrophotometry was then employed to analyze the EEOS compound. The known main active compounds were further analyzed for inhibitory and apoptotic effects on gene signaling using in silico molecular docking involving the downloading of active compounds from PubChem and target proteins from the Protein Data Bank; the active compounds and proteins were then prepared using the Discovery Studio software v. 19.0.0 and the PyRX 0.8 program, interacted with the HEX 8.0.0 program, and visualized with the Discovery Studio Visualizer v. 19.0. Finally, an in vitro analysis was performed using an antiproliferative-cytotoxic test (3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide assay in the NSCLC A549 cell line).ResultsThe analysis revealed that the active compounds in the ethanolic extract were dominated by quercetin (flavonoids) (47.23% b/b) and eugenol (phenolic) (12.14% b/b). These active compounds interacted with the active sites (residual amino acids) of the αvβ3 integrin, a5b1 integrin, caspase-3, caspase-9, and vascular endothelial growth factor. Hydrogen bonds and Pi-cation and Pi-alkyl interactions were involved in the relationships between the active compounds and the active sites and thus may reveal an antioxidant property of the extract. Furthermore, in vitro analysis showed the inhibitory and antiproliferative effects of the EEOS against non-small cell cancer (A549).ConclusionTaken together, our data showed the ability of EEOS as an inhibitor and apoptotic agent for lung cancer; however, further research is needed to determine the exact mechanism of EEOS as an herbal medication.

Project description:Endophytic mycopopulation isolated from India's Queen of herbs Tulsi (Ocimum sanctum) were explored and investigated for their diversity and antiphytopathogenic activity against widespread plant pathogens Botrytis cinerea, Sclerotinia sclerotiorum, Rhizoctonia solani and Fusarium oxysporum. 90 fungal isolates, representing 17 genera were recovered from 313 disease-free and surface sterilised plant segments (leaf and stem tissues) from three different geographic locations (Delhi, Hyderabad and Mukteshwar) during distinct sampling times in consequent years 2010 and 2011 in India. Fungal endophytes were subjected to molecular identification based on rDNA ITS sequence analysis. Plant pathogens such as F. verticillioides, B. maydis, C. coarctatum, R. bataticola, Hypoxylon sp., Diaporthe phaseolorum, Alternaria tenuissima and A. alternata have occurred as endophyte only during second sampling (second sampling in 2011) in the present study. Bi-plot generated by principal component analysis suggested tissue specificity of certain fungal endophytes. Dendrogram revealed species abundance as a function of mean temperature of the location at the time of sampling. Shannon diversity in the first collection is highest in Hyderabad leaf tissues (H' = 1.907) whereas in second collection it was highest from leaf tissues of Delhi (H' = 1.846). Mukteshwar (altitude: 7500 feet) reported least isolation rate in second collection. Nearly 23% of the total fungal isolates were considered as potent biocontrol agent. Hexane extract of M. phaseolina recovered from Hyderabad in first collection demonstrated highest activity against S. sclerotiorum with IC50 value of 0.38 mg/ml. Additionally, its components 2H-pyran-2-one, 5,6-dihydro-6-pentyl and palmitic acid, methyl ester as reported by GC-MS Chromatogram upon evaluation for their antiphytopathogenic activity exhibited IC50 value of 1.002 and 0.662 against respectively S. sclerotiorum indicating their significant role in antiphytopathogenic activity of hexane extract. The production of 2H-pyran-2-one, 5,6-dihydro-6-pentyl from M. phaseolina, an endophytic fungus is being reported for the first time.

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Data of The Expression of Serotonin in Alzheimer's Disease (AD) Rat Model Under Treatment of Ethanolic Extract Ocimum sanctum Linn.

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Data of The Expression of Serotonin in Alzheimer's Disease (AD) Rat Model Under Treatment of Ethanolic Extract Ocimum sanctum Linn.

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