Dataset Information

The effect of probe geometry on rind puncture resistance testing of maize stalks.

ABSTRACT: Background:Stalk lodging (breaking of plant stems prior to harvest) is a major impediment to increasing agricultural yields of grain crops. Rind puncture resistance is commonly used to predict the lodging resistance of several crop species. However, there exist no standard operating procedures or suggested protocols for conducting rind penetration experiments. In addition, experimental details of rind penetration tests such as the shape and size of the penetrating probe are rarely reported in the literature. This has prevented meta-analysis of results and has likewise prevented key findings of past studies from being replicated. As a first step towards establishing an agreed upon measurement standard for rind puncture resistance this study investigates the effect of the puncturing probe's geometry on test results. Results:Results demonstrate that probe geometry has a significant impact on test results. In particular, results showed that a 2 mm diameter chamfered probe produced stronger correlations with stalk bending strength than a 1.5 mm diameter pointed probe. The chamfered probe was also more strongly correlated with geometric features of the stalk that are known to influence stalk lodging resistance (e.g., rind thickness, diameter and section modulus). In addition, several alternative rind penetration metrics were investigated, and some were found to be superior to the most common rind penetration metric of maximum load. Conclusions:There is a need in the agricultural and plant science community to create agreed-upon operating procedures and testing standards related to mechanical traits of plant stems. In particular, a standardized probe geometry and insertion rate for rind penetration studies are needed to enable greater interoperability and meta-analysis of results. Probe shape and size should be reported in any study conducting rind penetration tests as these factors significantly impact test results.

SUBMITTER: Cook DD

PROVIDER: S-EPMC7206738 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

The effect of probe geometry on rind puncture resistance testing of maize stalks.

Cook Douglas D DD Meehan Kyler K Asatiani Levan L Robertson Daniel J DJ

Plant methods 20200508

<h4>Background</h4>Stalk lodging (breaking of plant stems prior to harvest) is a major impediment to increasing agricultural yields of grain crops. Rind puncture resistance is commonly used to predict the lodging resistance of several crop species. However, there exist no standard operating procedures or suggested protocols for conducting rind penetration experiments. In addition, experimental details of rind penetration tests such as the shape and size of the penetrating probe are rarely report ...[more]

PMID: 32411274

Similar Datasets

Project description:BackgroundStalk lodging (breaking of agricultural plant stalks prior to harvest) is a multi-billion dollar a year problem. Rind penetration resistance tests have been used by plant scientists and breeders to estimate the stalk lodging resistance of maize for nearly a hundred years. However, the rind puncture method has two key limitations: (1) the predictive power of the test decreases significantly when measuring elite or pre-commercial hybrids, and (2) using rind penetration measurements as a breeding metric does not necessarily create stronger stalks. In this study, we present a new rind penetration method called the Integrated Puncture Score, which uses a modified rind penetration testing protocol and a physics-based model to provide a robust measure of stalk lodging resistance.ResultsTwo datasets, one with a diverse array of maize hybrids and one with only elite hybrids, were evaluated by comparing traditional rind penetration testing and the Integrated Puncture Score method to measurements of stalk bending strength. When evaluating the diverse set of hybrids, both methods were good predictors of stalk bending strength (R2 values of 0.67). However, when evaluating elite hybrids, the Integrated Puncture Score had an R2 value of 0.74 whereas the traditional method had an R2 value of 0.48. Additionally, the Integrated Puncture Score was able to differentiate between the strongest and weakest hybrids in the elite hybrid data set whereas the traditional rind penetration method was not. Additional experiments revealed strong evidence in favor of the data aggregation steps utilized to compute the Integrated Puncture Score.ConclusionsThis study presents a new method for evaluating rind penetration resistance that highly correlates with stalk bending strength and can possibly be used as a breeding index for assessing stalk lodging resistance. This research lays the foundation required to develop a field-based high-throughput phenotyping device for stalk lodging resistance.

Project description:BackgroundMaize (Zea Mays L.) is one of the most important cereal crops worldwide and provides food for billions of people. Stalk lodging can greatly undermine the standability of maize plants and therefore decrease crop yields. Rind penetrometer resistance is an effective and reliable method for evaluating maize stalk strength, which is highly correlated with stalk lodging resistance. In this study, two recombinant inbred line populations were constructed from crosses between the H127R and Chang7-2 lines, and between the B73 and By804 lines. We genotyped these two populations and their parents using 3,072 single nucleotide polymorphism markers and performed phenotypic assessment of rind penetrometer resistance in multiple environments to dissect the genetic architecture of rind penetrometer resistance in maize.ResultsBased on two linkage maps of 1,397.1 and 1,600.4 cM with average interval of 1.7 and 2.1 cM between adjacent makers, respectively, seven quantitative trait loci (QTL) for rind penetrometer resistance were detected in the two recombinant inbred line populations. These QTL were distributed in seven genomic regions, and each accounted for 4.4-18.9% of the rind penetrometer resistance variation. The QTL with the largest effect on rind penetrometer resistance, qRPR3-1, was located on chromosome 3 with the flanking markers PZE-103123325 and SYN23245. This locus was further narrowed down to a 3.1-Mb interval by haplotype analysis using high-density markers in the target region. Within this interval, four genes associated with the biosynthesis of cell wall components were considered as potential candidate genes for the rind penetrometer resistance effect.ConclusionsThe inheritance of rind penetrometer resistance is rather complex. A few large-effect quantitative trait loci, together with a several minor-effect QTL, contributed to the phenotypic variation in rind penetrometer resistance in the two recombinant inbred line populations that were examined. A potential approach for improving stalk strength and crop yields in commercial maize lines may be to introgress favorable alleles of the locus that was found to have the largest effect on rind penetrometer resistance (qRPR3-1).

Dataset Information

The effect of probe geometry on rind puncture resistance testing of maize stalks.

Publications

The effect of probe geometry on rind puncture resistance testing of maize stalks.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets