A human lung tumor microenvironment interactome identifies clinically relevant cell-type cross-talk.

ABSTRACT:

Background

Tumors comprise a complex microenvironment of interacting malignant and stromal cell types. Much of our understanding of the tumor microenvironment comes from in vitro studies isolating the interactions between malignant cells and a single stromal cell type, often along a single pathway.

Result

To develop a deeper understanding of the interactions between cells within human lung tumors, we perform RNA-seq profiling of flow-sorted malignant cells, endothelial cells, immune cells, fibroblasts, and bulk cells from freshly resected human primary non-small-cell lung tumors. We map the cell-specific differential expression of prognostically associated secreted factors and cell surface genes, and computationally reconstruct cross-talk between these cell types to generate

SUBMITTER: Gentles AJ 

PROVIDER: S-EPMC7206807 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature