Dataset Information

Exploring the microbiome of healthy and diseased peri-implant sites using Illumina sequencing.

ABSTRACT: AIM:To compare the microbiome of healthy (H) and diseased (P) peri-implant sites and determine the core peri-implant microbiome. MATERIALS AND METHODS:Submucosal biofilms from 32 H and 35 P sites were analysed using 16S rRNA sequencing (MiSeq, Illumina), QIIME and HOMINGS. Differences between groups were determined using principal coordinate analysis (PCoA), t tests and Wilcoxon rank sum test and FDR-adjusted. The peri-implant core microbiome was determined. RESULTS:PCoA showed partitioning between H and P at all taxonomic levels. Bacteroidetes, Spirochetes and Synergistetes were higher in P, while Actinobacteria prevailed in H (p < .05). Porphyromonas and Treponema were more abundant in P while Rothia and Neisseria were higher in H (p < .05). The core peri-implant microbiome contained Fusobacterium, Parvimonas and Campylobacter sp. T. denticola, and P. gingivalis levels were higher in P, as well as F. alocis, F. fastidiosum and T. maltophilum (p < .05). CONCLUSION:The peri-implantitis microbiome is commensal-depleted and pathogen-enriched, harbouring traditional and new pathogens. The core peri-implant microbiome harbours taxa from genera often associated with periodontal inflammation.

SUBMITTER: Sanz-Martin I

PROVIDER: S-EPMC7209775 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature

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Exploring the microbiome of healthy and diseased peri-implant sites using Illumina sequencing.

Sanz-Martin Ignacio I Doolittle-Hall Janet J Teles Ricardo P RP Patel Michele M Belibasakis Georgios N GN Hämmerle Christoph H F CHF Jung Ronald E RE Teles Flavia R F FRF

Journal of clinical periodontology 20171121 12

<h4>Aim</h4>To compare the microbiome of healthy (H) and diseased (P) peri-implant sites and determine the core peri-implant microbiome.<h4>Materials and methods</h4>Submucosal biofilms from 32 H and 35 P sites were analysed using 16S rRNA sequencing (MiSeq, Illumina), QIIME and HOMINGS. Differences between groups were determined using principal coordinate analysis (PCoA), t tests and Wilcoxon rank sum test and FDR-adjusted. The peri-implant core microbiome was determined.<h4>Results</h4>PCoA sh ...[more]

PMID: 28766745

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Dataset Information

Exploring the microbiome of healthy and diseased peri-implant sites using Illumina sequencing.

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Exploring the microbiome of healthy and diseased peri-implant sites using Illumina sequencing.

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