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Analysis of the data on titration of native and peroxynitrite modified ?A- and ?B-crystallins by Cu2+-ions.

ABSTRACT: The interaction of ?A- and ?B-crystallins with Cu2+ ion modulates their structure and chaperone-like activity which is important for lens transparency. Theoretical analysis of the dependences of fluorescence intensity of native ?A- and ?B-crystallins and ?A- and ?B-crystallins modified by peroxynitrite on concentration of Cu2+ ions has been carried out. It has been shown that one subunit of native ?A-crystallin contains two equivalent Cu2+-binding sites. The microscopic dissociation constant for Cu2+-?A-crystallin complex (K diss) was found to be equal to 9.7 µM. For peroxynitrite modified ?A-crystallin the K diss value is equal to 17 µM. One subunit of native ?B-crystallin contains two non-equivalent Cu2+-binding sites. The corresponding microscopic dissociation constants for Cu2+-?B-crystallin complexes (K 1 and K 2) were found to be equal to 0.94 and 36 µM. For peroxynitrite modified ?B-crystallin the K 1 and K 2 values are equal to 4.3 and 70 µM, respectively.

SUBMITTER: Ghahramani M

PROVIDER: S-EPMC7210411 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature

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Analysis of the data on titration of native and peroxynitrite modified αA- and αB-crystallins by Cu2+-ions.

Ghahramani Maryam M Yousefi Reza R Khoshaman Kazem K Moghadam Sogand Sasan SS Kurganov Boris B

Data in brief 20200419

The interaction of αA- and αB-crystallins with Cu2+ ion modulates their structure and chaperone-like activity which is important for lens transparency. Theoretical analysis of the dependences of fluorescence intensity of native αA- and αB-crystallins and αA- and αB-crystallins modified by peroxynitrite on concentration of Cu2+ ions has been carried out. It has been shown that one subunit of native αA-crystallin contains two equivalent Cu2+-binding sites. The micr ...[more]

PMID: 32395573

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Project description:The treatment of wastewater containing heavy metals and the utilization of wool waste are very important for the sustainable development of textile mills. In this study, the wool keratin modified magnetite (Fe3O4) powders were fabricated by using wool waste via a co-precipitation technique for removal of Cu2+ ions from aqueous solutions. The morphology, chemical compositions, crystal structure, microstructure, magnetism properties, organic content, and specific surface area of as-fabricated powders were systematically characterized by various techniques including field emission scanning electron microscopy (FESEM), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), vibrating sample magnetometer (VSM), thermogravimetric (TG) analysis, and Brunauer-Emmett-Teller (BET) surface area analyzer. The effects of experimental parameters such as the volume of wool keratin hydrolysate, the dosage of powder, the initial Cu2+ ion concentration, and the pH value of solution on the adsorption capacity of Cu2+ ions by the powders were examined. The experimental results indicated that the Cu2+ ion adsorption performance of the wool keratin modified Fe3O4 powders exhibited much better than that of the chitosan modified ones with a maximum Cu2+ adsorption capacity of 27.4 mg/g under favorable conditions (0.05 g powders; 50 mL of 40 mg/L CuSO4; pH 5; temperature 293 K). The high adsorption capacity towards Cu2+ ions on the wool keratin modified Fe3O4 powders was primarily because of the strong surface complexation of -COOH and -NH2 functional groups of wool keratins with Cu2+ ions. The Cu2+ ion adsorption process on the wool keratin modified Fe3O4 powders followed the Temkin adsorption isotherm model and the intraparticle diffusion and pseudo-second-order adsorption kinetic models. After Cu2+ ion removal, the wool keratin modified Fe3O4 powders were easily separated using a magnet from aqueous solution and efficiently regenerated using 0.5 M ethylene diamine tetraacetic acid (EDTA)-H2SO4 eluting. The wool keratin modified Fe3O4 powders possessed good regenerative performance after five cycles. This study provided a feasible way to utilize waste wool textiles for preparing magnetic biomass-based adsorbents for the removal of heavy metal ions from aqueous solutions.

Dataset Information

Analysis of the data on titration of native and peroxynitrite modified ?A- and ?B-crystallins by Cu2+-ions.

Publications

Analysis of the data on titration of native and peroxynitrite modified αA- and αB-crystallins by Cu<sup>2+</sup>-ions.

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