Ontology highlight
ABSTRACT:
SUBMITTER: Elshenawy MM
PROVIDER: S-EPMC7212015 | biostudies-literature | 2020 May
REPOSITORIES: biostudies-literature
Elshenawy Mohamed M MM Kusakci Emre E Volz Sara S Baumbach Janina J Bullock Simon L SL Yildiz Ahmet A
Nature cell biology 20200427 5
Lissencephaly-1 (Lis1) is a key cofactor for dynein-mediated intracellular transport towards the minus-ends of microtubules. It remains unclear whether Lis1 serves as an inhibitor or an activator of mammalian dynein motility. Here we use single-molecule imaging and optical trapping to show that Lis1 does not directly alter the stepping and force production of individual dynein motors assembled with dynactin and a cargo adaptor. Instead, Lis1 promotes the formation of an active complex with dynac ...[more]