Purification of native CCL7 and its functional interaction with selected chemokine receptors.

ABSTRACT: Chemokine receptors form a major sub-family of G protein-coupled receptors (GPCRs) and they are involved in a number of cellular and physiological processes related to our immune response and regulation. A better structural understanding of ligand-binding, activation, signaling and regulation of chemokine receptors is very important to design potentially therapeutic interventions for human disorders arising from aberrant chemokine signaling. One of the key limitations in probing the structural details of chemokine receptors is the availability of large amounts of purified, homogenous and fully functional chemokine ligands, and the commercially available products, are not affordable for in-depth structural studies. Moreover, production of uniformly isotope-labeled chemokines, for example, s

SUBMITTER: Goncharuk MV 

PROVIDER: S-EPMC7212033 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature