The invasive proteome of glioblastoma revealed by laser-capture microdissection.
Ontology highlight
ABSTRACT: Background
Glioblastomas are heterogeneous tumors composed of a necrotic and tumor core and an invasive periphery.Methods
Here, we performed a proteomics analysis of laser-capture micro-dissected glioblastoma core and invasive areas of patient-derived xenografts.Results
Bioinformatics analysis identified enriched proteins in central and invasive tumor areas. Novel markers of invasion were identified, the genes proteolipid protein 1 (PLP1) and Dynamin-1 (DNM1), which were subsequently validated in tumors and by functional assays.Conclusions
In summary, our results identify new networks and molecules that may play an important role in glioblastoma development and may constitute potential novel therapeutic targets.
SUBMITTER: Daubon T
PROVIDER: S-EPMC7212852 | biostudies-literature | 2019 May-Dec
REPOSITORIES: biostudies-literature
Publications
The invasive proteome of glioblastoma revealed by laser-capture microdissection.
Daubon Thomas T Guyon Joris J Raymond Anne-Aurélie AA Dartigues Benjamin B Rudewicz Justine J Ezzoukhry Zakaria Z Dupuy Jean-William JW Herbert John M J JMJ Saltel Frédéric F Bjerkvig Rolf R Nikolski Macha M Bikfalvi Andreas A
Neuro-oncology advances 20190501 1
<h4>Background</h4>Glioblastomas are heterogeneous tumors composed of a necrotic and tumor core and an invasive periphery.<h4>Methods</h4>Here, we performed a proteomics analysis of laser-capture micro-dissected glioblastoma core and invasive areas of patient-derived xenografts.<h4>Results</h4>Bioinformatics analysis identified enriched proteins in central and invasive tumor areas. Novel markers of invasion were identified, the genes proteolipid protein 1 (PLP1) and Dynamin-1 (DNM1), which were ...[more]
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