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Effective coupling of rapid freeze-quench to high-frequency electron paramagnetic resonance.

ABSTRACT: We report an easy, efficient and reproducible way to prepare Rapid-Freeze-Quench samples in sub-millimeter capillaries and load these into the probe head of a 275 GHz Electron Paramagnetic Resonance spectrometer. Kinetic data obtained for the binding reaction of azide to myoglobin demonstrate the feasibility of the method for high-frequency EPR. Experiments on the same samples at 9.5 GHz show that only a single series of Rapid-Freeze-Quench samples is required for studies at multiple microwave frequencies.

SUBMITTER: Panarelli EG

PROVIDER: S-EPMC7213726 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Effective coupling of rapid freeze-quench to high-frequency electron paramagnetic resonance.

Panarelli E Gabriele EG van der Meer Harmen H Gast Peter P Groenen Edgar J J EJJ

PloS one 20200511 5

We report an easy, efficient and reproducible way to prepare Rapid-Freeze-Quench samples in sub-millimeter capillaries and load these into the probe head of a 275 GHz Electron Paramagnetic Resonance spectrometer. Kinetic data obtained for the binding reaction of azide to myoglobin demonstrate the feasibility of the method for high-frequency EPR. Experiments on the same samples at 9.5 GHz show that only a single series of Rapid-Freeze-Quench samples is required for studies at multiple microwave f ...[more]

PMID: 32392255

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