Functional culture and in vitro genetic and small-molecule manipulation of adult mouse cardiomyocytes.
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ABSTRACT:
SUBMITTER: Callaghan NI
PROVIDER: S-EPMC7214405 | biostudies-literature | 2020 May
REPOSITORIES: biostudies-literature
Publications
Functional culture and in vitro genetic and small-molecule manipulation of adult mouse cardiomyocytes.
Callaghan Neal I NI Lee Shin-Haw SH Hadipour-Lakmehsari Sina S Lee Xavier A XA Ahsan Siraj M M Driouchi Amine A Yip Christopher M CM Husain Mansoor M Simmons Craig A CA Gramolini Anthony O AO
Communications biology 20200511 1
Primary adult cardiomyocyte (aCM) represent the mature form of myocytes found in the adult heart. However, culture of aCMs in particular is challenged by poor survival and loss of phenotype, rendering extended in vitro experiments unfeasible. Here, we establish murine aCM culture methods that enhance survival and maintain sarcomeric structure and Ca<sup>2+</sup> cycling to enable physiologically relevant contractile force measurements. We also demonstrate genetic and small-molecule manipulations ...[more]
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