Dataset Information

Biocatalytic CO₂ Absorption and Structural Studies of Carbonic Anhydrase under Industrially-Relevant Conditions.

ABSTRACT: The unprecedently high CO₂ levels in the atmosphere evoke the urgent need for development of technologies for mitigation of its emissions. Among the alternatives, the biocatalytic route has been claimed as one of the most promising. In the present work, the carbonic anhydrase from bovine erythrocytes (BCA) was employed as a model enzyme for structural studies in an aqueous phase at alkaline pH, which is typical of large-scale absorption processes under operation. Circular dichroism (CD) analysis revealed a high enzymatic stability at pH 10 with a prominent decrease of the melting temperature above this value. The CO₂ absorption capacity of the aqueous solutions were assessed by online monitoring of pressure decay in a stainless-steel cell, which indicated a better performance at pH 10 with a kinetic rate increase of up to 43%, as compared to non-biocatalytic conditions. Even low enzyme concentrations (0.2 mg g^-1) proved to be sufficient to improve the overall CO₂ capture process performance. The enzyme-enhanced approach of CO₂ capture presents a high potential and should be further studied.

SUBMITTER: Castro AM

PROVIDER: S-EPMC7215295 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature

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Biocatalytic CO<sub>2</sub> Absorption and Structural Studies of Carbonic Anhydrase under Industrially-Relevant Conditions.

Castro Aline M de AM Ferreira Elisabete E Portugal Carla C Neves Luisa A LA Crespo João G JG

International journal of molecular sciences 20200422 8

The unprecedently high CO<sub>2</sub> levels in the atmosphere evoke the urgent need for development of technologies for mitigation of its emissions. Among the alternatives, the biocatalytic route has been claimed as one of the most promising. In the present work, the carbonic anhydrase from bovine erythrocytes (BCA) was employed as a model enzyme for structural studies in an aqueous phase at alkaline pH, which is typical of large-scale absorption processes under operation. Circular dichroism (C ...[more]

PMID: 32331206

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Project description:CO2 and bicarbonate are required for carboxylation reactions, which are essential in most bacteria. To provide the cells with sufficient CO2, there exist two dissolved inorganic carbon supply (DICS) systems: the membrane potential-generating system (MpsAB) and the carbonic anhydrase (CA). Recently, it has been shown that MpsAB is a bicarbonate transporter that is present not only in photo- and autotrophic bacteria, but also in a diverse range of nonautotrophic microorganisms. Since the two systems rarely coexist in a species but are interchangeable, we investigated what advantages the one system might have over the other. Using the genus Staphylococcus as a model, we deleted the CA gene can in Staphylococcus carnosus and mpsABC genes in Staphylococcus aureus. Deletion of the respective gene in one or the other species led to growth inhibition that could only be reversed by CO2 supplementation. While the S. carnosus Δcan mutant could be fully complemented with mpsABC, the S. aureus ΔmpsABC mutant was only partially complemented by can, suggesting that MpsAB outperforms CA. Interestingly, we provide evidence that mucus biofilm formation such as that involving polysaccharide intercellular adhesin (PIA) impedes the diffusion of CO2 into cells, making MpsAB more advantageous in biofilm-producing strains or species. Coexpression of MpsAB and CA does not confer any growth benefits, even under stress conditions. In conclusion, the distribution of MpsAB or CA in bacteria does not appear to be random as expression of bicarbonate transporters provides an advantage where diffusion of CO2 is impeded. IMPORTANCE CO2 and bicarbonate are required for carboxylation reactions in central metabolism and biosynthesis of small molecules in all bacteria. This is achieved by two different systems for dissolved inorganic carbon supply (DICS): these are the membrane potential-generating system (MpsAB) and the carbonic anhydrase (CA), but both rarely coexist in a given species. Here, we compared both systems and demonstrate that the distribution of MpsAB and/or CA within the phylum Firmicutes is apparently not random. The bicarbonate transporter MpsAB has an advantage in species where CO2 diffusion is hampered-for instance, in mucus- and biofilm-forming bacteria. However, coexpression of MpsAB and CA does not confer any growth benefits, even under stress conditions. Given the clinical relevance of Staphylococcus in the medical environment, such findings contribute to the understanding of bacterial metabolism and thus are crucial for exploration of potential targets for antimicrobials. The knowledge gained here as exemplified by staphylococcal species could be extended to other pathogenic bacteria.

Dataset Information

Biocatalytic CO₂ Absorption and Structural Studies of Carbonic Anhydrase under Industrially-Relevant Conditions.

Publications

Biocatalytic CO<sub>2</sub> Absorption and Structural Studies of Carbonic Anhydrase under Industrially-Relevant Conditions.

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Dataset Information

Biocatalytic CO2 Absorption and Structural Studies of Carbonic Anhydrase under Industrially-Relevant Conditions.

Publications

Biocatalytic CO<sub>2</sub> Absorption and Structural Studies of Carbonic Anhydrase under Industrially-Relevant Conditions.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

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Biocatalytic CO₂ Absorption and Structural Studies of Carbonic Anhydrase under Industrially-Relevant Conditions.