RT-qPCR Testing of SARS-CoV-2: A Primer.
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ABSTRACT:
SUBMITTER: Bustin SA
PROVIDER: S-EPMC7215906 | biostudies-literature | 2020 Apr
REPOSITORIES: biostudies-literature
Publications
RT-qPCR Testing of SARS-CoV-2: A Primer.
Bustin Stephen A SA Nolan Tania T
International journal of molecular sciences 20200424 8
Testing for the presence of coronavirus is an essential diagnostic tool for monitoring and managing the current COVID-19 pandemic. The only reliable test in current use for testing acute infection targets the genome of SARS-CoV-2, and the most widely used method is quantitative fluorescence-based reverse transcription polymerase chain reaction (RT-qPCR). Despite its ubiquity, there is a significant amount of uncertainty about how this test works, potential throughput and reliability. This has re ...[more]
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