Dataset Information

Improving Azo Dye Decolorization Performance and Halotolerance of Pichia occidentalis A2 by Static Magnetic Field and Possible Mechanisms Through Comparative Transcriptome Analysis.

ABSTRACT: A halotolerant yeast, Pichia occidentalis A2, was recently isolated that can decolorize various azo dyes. The azo dye decolorization performance of this strain was characterized, including the degradation pathway and detoxification effects of this yeast. Additionally, the effect of static magnetic field (SMF) on this decolorization process was investigated. Activities of key enzymes were analyzed to estimate the change of metabolic activity. Furthermore, possible mechanisms were analyzed through detecting differentially expressed genes between yeast A2 in the absence and presence of SMF. The results indicated that yeast A2 displayed the optimal decolorization performance when the concentrations (in g/L) of glucose, (NH4)2SO4, yeast extract, and NaCl were 4.0, 1.0, 0.1, and ?30.0, respectively. Meanwhile, the optimal rotation speed, temperature, and pH were 160 rpm, 30°C, and 5.0, respectively. Acid Red B was decolorized and detoxified by yeast A2 through successive steps, including cleavage of the naphthalene-amidine bond, reductive deamination, oxidative deamination/desulfurization, open-loop of hydroxy-substituted naphthalene, and tricarboxylic acid cycle. The dye decolorization efficiency and halotolerance of yeast A2 were enhanced by 206.3 mT SMF. The activities of manganese peroxidase, and laccase were elevated 1.37- and 1.16-fold by 206.3 mT SMF, but lignin peroxidase activity showed little change. It was suggested from the transcriptome sequence that the enhanced halotolerance might be related to the upregulated genes encoding the enzymes or functional proteins related to intracellular synthesis and accumulation of glycerol.

SUBMITTER: Wang X

PROVIDER: S-EPMC7216737 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Improving Azo Dye Decolorization Performance and Halotolerance of <i>Pichia occidentalis</i> A2 by Static Magnetic Field and Possible Mechanisms Through Comparative Transcriptome Analysis.

Wang Xiaohan X Wang Yumeng Y Ning Shuxiang S Shi Shengnan S Tan Liang L

Frontiers in microbiology 20200505

A halotolerant yeast, <i>Pichia occidentalis</i> A2, was recently isolated that can decolorize various azo dyes. The azo dye decolorization performance of this strain was characterized, including the degradation pathway and detoxification effects of this yeast. Additionally, the effect of static magnetic field (SMF) on this decolorization process was investigated. Activities of key enzymes were analyzed to estimate the change of metabolic activity. Furthermore, possible mechanisms were analyzed ...[more]

PMID: 32431675

Similar Datasets

Project description:Textile dyes are one of the major water pollutants released into water in various ways, posing serious hazards for both aquatic organisms and human beings. Bioremediation is a significantly promising technique for dye decolorization. In the present study, the fungal strain Lasiodiplodia sp. was isolated from the fruiting bodies of Schizophyllum for the first time. The isolated fungal strain was examined for laccase enzyme production under solid-state fermentation conditions with wheat bran (WB) using ABTS and 2,6-Dimethoxyphenol (DMP) as substrates, then the fermented wheat bran (FWB) was evaluated as a biosorbent for Congo red dye adsorption from aqueous solutions in comparison with unfermented wheat bran. A Box-Behnken design was used to optimize the dye removal by FWB and to analyze the interaction effects between three factors: fermentation duration, pH, and dye concentration. Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and scanning electron microscopy (SEM) were applied to study the changes in the physical and chemical characteristics of wheat bran before and after fermentation. An additional experiment was conducted to investigate the ability of the Lasiodiplodia sp. YZH1 to remove Congo red in the dye-containing liquid culture. The results showed that laccase was produced throughout the cultivation, reaching peak activities of ∼6.2 and 22.3 U/mL for ABTS and DMP, respectively, on the fourth day of cultivation. FWB removed 89.8% of the dye (100 mg L-1) from the aqueous solution after 12 h of contact, whereas WB removed only 77.5%. Based on the Box-Behnken design results, FWB achieved 93.08% dye removal percentage under the conditions of 6 days of fermentation, pH 8.5, and 150 mg L-1 of the dye concentration after 24 h. The fungal strain removed 95.3% of 150 mg L-1 of the dye concentration after 8 days of inoculation in the dye-containing liquid culture. These findings indicate that this strain is a worthy candidate for dye removal from environmental effluents.

Project description:The effective degradation of hazardous contaminants remains an intractable challenge in wastewater processing, especially for the high concentration of salty azo dye wastewater. However, some unique yeast symbionts identified from the termite gut system present an impressive function to deconstruct some aromatic compounds, which imply that they may be valued to work on the dye degradation for various textile effluents. In this investigation, a newly isolated and unique yeast strain, Sterigmatomyces halophilus SSA-1575, was identified from the gut system of a wood-feeding termite (WFT), Reticulitermes chinensis. Under the optimized ambient conditions, the yeast strain SSA-1575 showed a complete decolorization efficiency on Reactive Black 5 (RB5) within 24 h, where this azo dye solution had a concentration of a 50 mg/L RB5. NADH-dichlorophenol indophenol (NADH-DCIP) reductase and lignin peroxidase (LiP) were determined as the key reductase and oxidase of S. halophilus SSA-1575. Enhanced decolorization was recorded when the medium was supplemented with carbon and energy sources, including glucose, ammonium sulfate, and yeast extract. To understand a possible degradation pathway well, UV-Vis spectroscopy, FTIR and Mass Spectrometry analyses were employed to analyze the possible decolorization pathway by SSA-1575. Determination of relatively high NADH-DCIP reductase suggested that the asymmetric cleavage of RB5 azo bond was mainly catalyzed by NADH-DCIP reductase, and finally resulting in the formation of colorless aromatic amines devoid of any chromophores. The ecotoxicology assessment of RB5 after a decolorization processing by SSA-1575, was finally conducted to evaluate the safety of its metabolic intermediates from RB5. The results of Microtox assay indicate a capability of S. halophilus SSA-1575, in the detoxification of the toxic RB5 pollutant. This study revealed the effectiveness of halotolerant yeasts in the eco-friendly remediation of hazardous pollutants and dye wastewater processing for the textile industry.

Dataset Information

Improving Azo Dye Decolorization Performance and Halotolerance of Pichia occidentalis A2 by Static Magnetic Field and Possible Mechanisms Through Comparative Transcriptome Analysis.

Publications

Improving Azo Dye Decolorization Performance and Halotolerance of <i>Pichia occidentalis</i> A2 by Static Magnetic Field and Possible Mechanisms Through Comparative Transcriptome Analysis.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets