Dataset Information

Enzymatic Hydrolysis of Bacterial Cellulose for the Production of Nanocrystals for the Food Packaging Industry.

ABSTRACT: Bacterial cellulose nanocrystals (BCNCs) obtained by enzymatic hydrolysis have been loaded in pullulan biopolymer for use as nanoparticles in the generation of high-oxygen barrier coatings intended for food packaging applications. Bacterial cellulose (BC) produced by Komagataeibacter sucrofermentans was hydrolyzed by two different enzymatic treatments, i.e., using endo-1,4-?-glucanases (EGs) from Thermobifida halotolerans and cellulase from Trichoderma reesei. The hydrolytic activity was compared by means of turbidity experiments over a period of 145 h, whereas BCNCs in their final state were compared, in terms of size and morphology, by atomic force microscopy (AFM) and dynamic light scattering (DLS). Though both treatments led to particles of similar size, a greater amount of nano-sized particles (?250 nm) were observed in the system that also included cellulase enzymes. Unexpectedly, transmission electron microscopy (TEM) revealed that cellulose nanoparticles were round-shaped and made of 4-5 short (150-180 nm) piled whiskers. Pullulan/BCNCs nanocomposite coatings allowed an increase in the overall oxygen barrier performance, of more than two and one orders of magnitude (?0.7 mL·m-2·24 h-1), of pure polyethylene terephthalate (PET) (?120 mL·m-2·24 h-1) as well as pullulan/coated PET (?6 mL·m-2·24 h-1), with no significant difference between treatments (hydrolysis mediated by EGs or with the addition of cellulase). BCNCs obtained by enzymatic hydrolysis have the potential to generate high oxygen barrier coatings for the food packaging industry.

SUBMITTER: Rovera C

PROVIDER: S-EPMC7221805 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Enzymatic Hydrolysis of Bacterial Cellulose for the Production of Nanocrystals for the Food Packaging Industry.

Rovera Cesare C Fiori Filippo F Trabattoni Silvia S Romano Diego D Farris Stefano S

Nanomaterials (Basel, Switzerland) 20200411 4

Bacterial cellulose nanocrystals (BCNCs) obtained by enzymatic hydrolysis have been loaded in pullulan biopolymer for use as nanoparticles in the generation of high-oxygen barrier coatings intended for food packaging applications. Bacterial cellulose (BC) produced by Komagataeibacter sucrofermentans was hydrolyzed by two different enzymatic treatments, i.e., using endo-1,4-β-glucanases (EGs) from Thermobifida halotolerans and cellulase from Trichoderma reesei. The hydrolytic ...[more]

PMID: 32290503

Similar Datasets

Project description:BackgroundDuring cellulosic ethanol production, cellulose hydrolysis is achieved by synergistic action of cellulase enzyme complex consisting of multiple enzymes with different mode of actions. Enzymatic hydrolysis of cellulose is one of the bottlenecks in the commercialization of the process due to low hydrolysis rates and high cost of enzymes. A robust hydrolysis model that can predict hydrolysis profile under various scenarios can act as an important forecasting tool to improve the hydrolysis process. However, multiple factors affecting hydrolysis: cellulose structure and complex enzyme-substrate interactions during hydrolysis make it diffucult to develop mathematical kinetic models that can simulate hydrolysis in presence of multiple enzymes with high fidelity. In this study, a comprehensive hydrolysis model based on stochastic molecular modeling approch in which each hydrolysis event is translated into a discrete event is presented. The model captures the structural features of cellulose, enzyme properties (mode of actions, synergism, inhibition), and most importantly dynamic morphological changes in the substrate that directly affect the enzyme-substrate interactions during hydrolysis.ResultsCellulose was modeled as a group of microfibrils consisting of elementary fibrils bundles, where each elementary fibril was represented as a three dimensional matrix of glucose molecules. Hydrolysis of cellulose was simulated based on Monte Carlo simulation technique. Cellulose hydrolysis results predicted by model simulations agree well with the experimental data from literature. Coefficients of determination for model predictions and experimental values were in the range of 0.75 to 0.96 for Avicel hydrolysis by CBH I action. Model was able to simulate the synergistic action of multiple enzymes during hydrolysis. The model simulations captured the important experimental observations: effect of structural properties, enzyme inhibition and enzyme loadings on the hydrolysis and degree of synergism among enzymes.ConclusionsThe model was effective in capturing the dynamic behavior of cellulose hydrolysis during action of individual as well as multiple cellulases. Simulations were in qualitative and quantitative agreement with experimental data. Several experimentally observed phenomena were simulated without the need for any additional assumptions or parameter changes and confirmed the validity of using the stochastic molecular modeling approach to quantitatively and qualitatively describe the cellulose hydrolysis.

Project description:Cellulosic biowastes are one of the cheapest and most abundant renewable organic materials on earth that can be, subsequent to hydrolysis, utilized as an organic carbon source for several fermentation biotechnologies. This study was devoted to explore a semidry acid hydrolysis of cellulose for decreasing the cost and ionic strength of the hydrolysate. For semidry acid hydrolysis, cellulose was just wetted with HCl (0 to 7 M) and subjected to autoclaving. The optimum molar concentration of HCl and period of autoclaving for semidry acid hydrolysis of cellulose were 6 M and 50 min respectively. Subsequent to the semidry acid hydrolysis with a minimum volume of 6 M HCl sustained by autoclaving, the hydrolysate was diluted with distilled water and neutralized with NaOH (0.5 M). The reducing sugars produced from the semidry acid hydrolysis of cellulose was further used for dark fermentation biohydrogen production by Escherichia coli as a representative of most hydrogen producing eubacteria which cannot utilize non-hydrolyzed cellulose. An isolated E. coli TFYM was used where this bacterium was morphologically and biochemically characterized and further identified by phylogenetic 16S rRNA encoding gene sequence analysis. The reducing sugars produced by semidry acid hydrolysis could be efficiently utilized by E. coli producing 0.4 mol H2 mol-1 hexose with a maximum rate of hydrogen gas production of 23.3 ml H2 h-1 L-1 and an estimated hydrogen yield of 20.5 (L H2 kg-1 dry biomass). The cheap cellulosic biowastes of wheat bran, sawdust and sugarcane bagasse could be hydrolyzed by semidry acid hydrolysis where the estimated hydrogen yield per kg of its dry biomass were 36, 18 and 32 (L H2 kg-1 dry biomass) respectively indicating a good feasibility of hydrogen production from reducing sugars prepared by semidry acid hydrolysis of these cellulosic biowastes. Semidry acid hydrolysis could also be effectively used for hydrolyzing non-cellulosic polysaccharides of dry cyanobacterial biomass. The described semidry acid hydrolysis of cellulosic biowastes in this study might be applicable not only for bacterial biohydrogen production but also for various hydrolyzed cellulose-based fermentation biotechnologies.

Dataset Information

Enzymatic Hydrolysis of Bacterial Cellulose for the Production of Nanocrystals for the Food Packaging Industry.

Publications

Enzymatic Hydrolysis of Bacterial Cellulose for the Production of Nanocrystals for the Food Packaging Industry.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets