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First-Pass Metabolism of Polyphenols from Selected Berries: A High-Throughput Bioanalytical Approach.


ABSTRACT: Small berries are rich in polyphenols whose first-pass metabolism may alter their ultimate physiological effects. The antioxidant capacity and polyphenol profile of three freeze-dried berries (blackberry, raspberry, Red Globe grape) were measured and their apparent permeability (Papp) and first-pass biotransformation were tracked with an ex vivo bioanalytical system [everted gut sac (rat) + three detection methods: spectrophotometry, HPLC-ESI-QTOF-MS, differential pulse voltammetry (DPV)]. Total polyphenol (ratio 0.07-0.14-1.0) and molecular diversity (anthocyanins>flavan-3-ols), antioxidant capacity (DPPH, FRAP), anodic current maxima and Papp (efflux> uptake) were in the following order: blackberry > raspberry > Red Globe grape. Epicatechin, pelargonidin & cyanin (all), callistephin (raspberry/blackberry), catechin (grape), cyanidin glycosides (blackberry) and their derived metabolites [quinic acid, epicatechin, cyanidin/malvidin glucosides, and chlorogenic/caffeic acids] were fruit-specific and concentration-dependent. Time-trend DPV kinetic data revealed concurrent epithelial permeability & biotransformation processes. Regular permeability and high-biotransformation of berry polyphenols suggest fruit-specific health effects apparently at the intestinal level.

SUBMITTER: Olivas-Aguirre FJ 

PROVIDER: S-EPMC7222205 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature

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First-Pass Metabolism of Polyphenols from Selected Berries: A High-Throughput Bioanalytical Approach.

Olivas-Aguirre Francisco J FJ   Mendoza Sandra S   Alvarez-Parrilla Emilio E   Gonzalez-Aguilar Gustavo A GA   Villegas-Ochoa Monica A MA   Quintero-Vargas Jael T J JTJ   Wall-Medrano Abraham A  

Antioxidants (Basel, Switzerland) 20200413 4


Small berries are rich in polyphenols whose first-pass metabolism may alter their ultimate physiological effects. The antioxidant capacity and polyphenol profile of three freeze-dried berries (blackberry, raspberry, Red Globe grape) were measured and their apparent permeability (Papp) and first-pass biotransformation were tracked with an ex vivo bioanalytical system [everted gut sac (rat) + three detection methods: spectrophotometry, HPLC-ESI-QTOF-MS, differential pulse voltammetry (DPV)]. Total  ...[more]

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