Dataset Information

Genome Assembly of Azotobacter chroococcum Strain W5, a Free-Living Diazotroph Isolated from India.

ABSTRACT: Azotobacter chroococcum strain W5 (MTCC 25045) is an effective diazotrophic bacterium with plant growth-promoting traits. Here, we report the draft genome assembly of this biologically and agronomically evaluated A. chroococcum strain. The genome assembly in 55 contigs is 4,617,864?bp long, with a G+C content of 66.83%.

SUBMITTER: Kannepalli A

PROVIDER: S-EPMC7225537 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature

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Genome Assembly of Azotobacter chroococcum Strain W5, a Free-Living Diazotroph Isolated from India.

Kannepalli Annapurna A Pengani Kedharnath Reddy KR Yasin Jeshima Khan JK Paul Sangeeta S Tyagi Swati S Venkadasamy Govindasamy G Sharma Meenakshi M Karivaradharajan Swarnalakshmi S

Microbiology resource announcements 20200514 20

<i>Azotobacter chroococcum</i> strain W5 (MTCC 25045) is an effective diazotrophic bacterium with plant growth-promoting traits. Here, we report the draft genome assembly of this biologically and agronomically evaluated <i>A. chroococcum</i> strain. The genome assembly in 55 contigs is 4,617,864 bp long, with a G+C content of 66.83%. ...[more]

PMID: 32409538

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Project description:The genome of the soil-dwelling heterotrophic N2-fixing Gram-negative bacterium Azotobacter chroococcum NCIMB 8003 (ATCC 4412) (Ac-8003) has been determined. It consists of 7 circular replicons totalling 5,192,291 bp comprising a circular chromosome of 4,591,803 bp and six plasmids pAcX50a, b, c, d, e, f of 10,435 bp, 13,852, 62,783, 69,713, 132,724, and 311,724 bp respectively. The chromosome has a G+C content of 66.27% and the six plasmids have G+C contents of 58.1, 55.3, 56.7, 59.2, 61.9, and 62.6% respectively. The methylome has also been determined and 5 methylation motifs have been identified. The genome also contains a very high number of transposase/inactivated transposase genes from at least 12 of the 17 recognised insertion sequence families. The Ac-8003 genome has been compared with that of Azotobacter vinelandii ATCC BAA-1303 (Av-DJ), a derivative of strain O, the only other member of the Azotobacteraceae determined so far which has a single chromosome of 5,365,318 bp and no plasmids. The chromosomes show significant stretches of synteny throughout but also reveal a history of many deletion/insertion events. The Ac-8003 genome encodes 4628 predicted protein-encoding genes of which 568 (12.2%) are plasmid borne. 3048 (65%) of these show > 85% identity to the 5050 protein-encoding genes identified in Av-DJ, and of these 99 are plasmid-borne. The core biosynthetic and metabolic pathways and macromolecular architectures and machineries of these organisms appear largely conserved including genes for CO-dehydrogenase, formate dehydrogenase and a soluble NiFe-hydrogenase. The genetic bases for many of the detailed phenotypic differences reported for these organisms have also been identified. Also many other potential phenotypic differences have been uncovered. Properties endowed by the plasmids are described including the presence of an entire aerobic corrin synthesis pathway in pAcX50f and the presence of genes for retro-conjugation in pAcX50c. All these findings are related to the potentially different environmental niches from which these organisms were isolated and to emerging theories about how microbes contribute to their communities.

Dataset Information

Genome Assembly of Azotobacter chroococcum Strain W5, a Free-Living Diazotroph Isolated from India.

Publications

Genome Assembly of Azotobacter chroococcum Strain W5, a Free-Living Diazotroph Isolated from India.

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