Ontology highlight
ABSTRACT:
SUBMITTER: Singh A
PROVIDER: S-EPMC7225587 | biostudies-literature | 2020
REPOSITORIES: biostudies-literature
Singh Anupam A Upadhyay Vaibhav V Singh Akansha A Panda Amulya K AK
Frontiers in microbiology 20200508
High level expression of recombinant proteins in bacteria often results in their aggregation into inclusion bodies. Formation of inclusion bodies poses a major bottleneck in high-throughput recovery of recombinant protein. These aggregates have amyloid-like nature and can retain biological activity. Here, effect of expression temperature on the quality of <i>Escherichia coli</i> asparaginase II (a tetrameric protein) inclusion bodies was evaluated. Asparaginase was expressed as inclusion bodies ...[more]