Ontology highlight
ABSTRACT:
SUBMITTER: Zhang Q
PROVIDER: S-EPMC7225609 | biostudies-literature | 2020
REPOSITORIES: biostudies-literature
Zhang Qiang Q Guo Yaqiong Y Li Na N Li Yu Y Su Jiayuan J Xu Rui R Zhang Ziding Z Feng Yaoyu Y Xiao Lihua L
Frontiers in microbiology 20200508
Calcium-dependent protein kinases (CDPKs) are considered promising targets for pharmaceutical intervention of cryptosporidiosis. Whole-genome sequencing has revealed the presence of several CDPKs (<i>Cp</i>CDPKs) in <i>Cryptosporidium parvum</i>. In this study, we expressed recombinant <i>Cp</i>CDPK3 encoded by the <i>cgd5_820</i> gene in <i>Escherichia coli</i>. The biologic characteristics and functions of <i>Cp</i>CDPK3 were examined using qRT-PCR, immunofluorescence microscopy, and <i>in vit ...[more]