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High Expression of the Sd^a Synthase B4GALNT2 Associates with Good Prognosis and Attenuates Stemness in Colon Cancer.

ABSTRACT:

Background

The carbohydrate antigen Sd^a and its biosynthetic enzyme B4GALNT2 are highly expressed in normal colonic mucosa but are down-regulated to a variable degree in colon cancer tissues. Here, we investigated the clinical and biological importance of B4GALNT2 in colon cancer.

Methods

Correlations of B4GALNT2 mRNA with clinical data were obtained from The Cancer Genome Atlas (TCGA) database; the phenotypic and transcriptomic changes induced by B4GALNT2 were studied in LS174T cells transfected with B4GALNT2 cDNA.

Results

TCGA data indicate that patients with high B4GALNT2 expression in cancer tissues display longer survival than non-expressers. In LS174T cells, expression of B4GALNT2 did not affect the ability to heal a scratch wound or to form colonies in standard growth conditions but markedly reduced the growth in soft agar, the tridimensional (3D) growth as spheroids, and the number of cancer stem cells, indicating a specific effect of B4GALNT2 on the growth in poor adherence and stemness. On the transcriptome, B4GALNT2 induced the down-regulation of the stemness-associated gene SOX2 and modulated gene expression towards an attenuation of the cancer phenotype.

Conclusions

The level of B4GALNT2 can be proposed as a marker to identify higher- and lower-risk colorectal cancer patients.

SUBMITTER: Pucci M

PROVIDER: S-EPMC7226961 | biostudies-literature |

REPOSITORIES: biostudies-literature

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