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Trapping and Manipulation of Single Cells in Crowded Environments.

ABSTRACT: Optical tweezers provide a powerful tool to trap and manipulate living cells, which is expected to help people gain physiological insights at single-cell level. However, trapping and manipulating single cells under crowded environments, such as blood vessels and lymph nodes, is still a challenging task. To overcome this issue, an annular beam formed by the far-field Bessel beam is introduced to serve as an optical shield to isolate the target cells from being disturbed. With this scheme, we successfully trapped and manipulated single blood cells in a crowded environment. Furthermore, we demonstrated manipulation of two lymphocytes ejected from a lymph node independently with dual-trap optical tweezers, which paves the way for exploring cell interactions under living conditions. Such technique might be helpful in the study of how natural killer cells response to virus-infected cells or cancer cells.

SUBMITTER: Zhao Q

PROVIDER: S-EPMC7227430 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Trapping and Manipulation of Single Cells in Crowded Environments.

Zhao Qian Q Wang Hao-Wei HW Yu Pan-Pan PP Zhang Shu-He SH Zhou Jin-Hua JH Li Yin-Mei YM Gong Lei L

Frontiers in bioengineering and biotechnology 20200508

Optical tweezers provide a powerful tool to trap and manipulate living cells, which is expected to help people gain physiological insights at single-cell level. However, trapping and manipulating single cells under crowded environments, such as blood vessels and lymph nodes, is still a challenging task. To overcome this issue, an annular beam formed by the far-field Bessel beam is introduced to serve as an optical shield to isolate the target cells from being disturbed. With this scheme, we succ ...[more]

PMID: 32457890

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