Dataset Information

Adsorption of Deoxynivalenol (DON) from Corn Steep Liquor (CSL) by the Microsphere Adsorbent SA/CMC Loaded with Calcium.

ABSTRACT: The occurrence of deoxynivalenol (DON) in animal feed is a serious issue for the livestock industry. Approaches using mycotoxin adsorbents are key to decreasing mycotoxin carryover from contaminated feed to animals. In this paper, a novel functional microsphere adsorbent comprising an alginate/carboxymethyl cellulose sodium composite loaded with calcium (SA/CMC-Ca) was prepared by an emulsification process to adsorb DON from polluted corn steep liquor (CSL) containing DON at a concentration of 3.60 ?g/mL. Batch experiments were conducted under different experimental conditions: CSL volumes, reaction times, desorption times, and microsphere recyclability. Results showed that 5 g of microspheres reacted with 5 mL of DON-polluted CSL for 5 min, the microspheres can be recycled 155 times, and the maximum DON adsorption for the microspheres was 2.34 ?g/mL. During recycling, microspheres were regenerated by deionized water every time; after the microspheres were cleaned, DON in the deionized water was degraded by sodium hydroxide (NaOH) at 70 °C for 1 h at pH 12. The mechanism for physical adsorption and hydrogen bonding was analyzed by scanning electron microscopy (SEM) and Fourier transform infrared spectrometry (FTIR). To the best of our knowledge, this is the first report showing that the microsphere adsorbent SA/CMC-Ca adsorbs DON. Therefore, we suggest that using microsphere absorbents would be a possible way to address DON-contaminated CSL issues in animal feed.

SUBMITTER: Shalapy A

PROVIDER: S-EPMC7232427 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature

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Adsorption of Deoxynivalenol (DON) from Corn Steep Liquor (CSL) by the Microsphere Adsorbent SA/CMC Loaded with Calcium.

Shalapy Ahmed A Zhao Shuangqing S Zhang Chenxi C Li Yifei Y Geng Hairong H Ullah Sana S Wang Gang G Huang Shujian S Liu Yang Y

Toxins 20200325 4

The occurrence of deoxynivalenol (DON) in animal feed is a serious issue for the livestock industry. Approaches using mycotoxin adsorbents are key to decreasing mycotoxin carryover from contaminated feed to animals. In this paper, a novel functional microsphere adsorbent comprising an alginate/carboxymethyl cellulose sodium composite loaded with calcium (SA/CMC-Ca) was prepared by an emulsification process to adsorb DON from polluted corn steep liquor (CSL) containing DON at a concentration of 3 ...[more]

PMID: 32218143

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Project description:BackgroundCarbon and nitrogen are essential energy and nutrient substances in the composting process. Corn steep liquor (CSL) is rich in soluble carbon and nitrogen nutrients and active substances and is widely used in the biological industry. Nonetheless, limited research has been done on the effect of CSL on composting. This work firstly reveals the effect of adding CSL to bacterial community composition and carbon and nitrogen conversion during composting. This study provides the choice of auxiliary materials for the spent mushroom substrate compost (SMS) and some novel knowledge about the effect of bacterial community on C and N cycling during composting of SMS and CSL. Two treatments were set up in the experiment: 100% spent mushroom substrate (SMS) as CK and SMS + 0.5% CSL (v/v) as CP.ResultsThe results showed that the addition of CSL enhanced the initial carbon and nitrogen content of the compost, altered the bacterial community structure, and increased the bacterial diversity and relative abundance, which might be beneficial to the conversion and retention of carbon and nitrogen in the composting process. In this paper, network analysis was used to screen the core bacteria involved in carbon and nitrogen conversion. In the CP network, the core bacteria were divided into two categories, synthesizing and degrading bacteria, and there were more synthesizing bacteria than degrading bacteria, so the degradation and synthesis of organic matter were carried out simultaneously, while only degrading bacteria were found in the CK network. Functional prediction by Faprotax identified 53 groups of functional bacteria, among which 20 (76.68% abundance) and 14 (13.15% abundance) groups of functional bacteria were related to carbon and nitrogen conversion, respectively. Adding CSL stimulated the compensatory effect of core and functional bacteria, enhanced the carbon and nitrogen transformation ability, stimulated the activity of low-abundance bacteria, and reduced the competitive relationship between the bacterial groups. This may be why the addition of CSL accelerated the organic matter degradation and increased carbon and nitrogen preservation.ConclusionsThese findings indicate that the addition of CSL promoted the cycling and preservation of carbon and nitrogen in the SMS composts, and the addition of CSL to the compost may be an effective way to dispose of agricultural waste.

Project description:The Fusarium mycotoxin deoxynivalenol (DON) is a frequent contaminant of cereal-based food and feed. Mammals metabolize DON by conjugation to glucuronic acid (GlcAc), the extent and regioselectivity of which is species-dependent. So far, only DON-3-glucuronide (DON-3-GlcAc) and DON-15-GlcAc have been unequivocally identified as mammalian DON glucuronides, and DON-7-GlcAc has been proposed as further DON metabolite. In the present work, qualitative HPLC-MS/MS analysis of urine samples of animals treated with DON (rats: 2 mg/kg bw, single bolus, gavage; mice: 1 mg/kg bw, single i.p. injection; pigs: 74 µg/kg bw, single bolus, gavage; cows: 5.2 mg DON/kg dry mass, oral for 13 weeks) revealed additional DON and deepoxy-DON (DOM) glucuronides. To elucidate their structures, DON and DOM were incubated with human (HLM) and rat liver microsomes (RLM). Besides the expected DON/DOM-3- and 15-GlcAc, minor amounts of four DON- and four DOM glucuronides were formed. Isolation and enzymatic hydrolysis of four of these compounds yielded iso-DON and iso-DOM, the identities of which were eventually confirmed by NMR. Incubation of iso-DON and iso-DOM with RLM and HLM yielded two main glucuronides for each parent compound, which were isolated and identified as iso-DON/DOM-3-GlcAc and iso-DON/DOM-8-GlcAc by NMR. Iso-DON-3-GlcAc, most likely misidentified as DON-7-GlcAc in the literature, proved to be a major DON metabolite in rats and a minor metabolite in pigs. In addition, iso-DON-8-GlcAc turned out to be one of the major DON metabolites in mice. DOM-3-GlcAc was the dominant DON metabolite in urine of cows and an important DON metabolite in rat urine. Iso-DOM-3-GlcAc was detected in urine of DON-treated rats and cows. Finally, DON-8,15-hemiketal-8-glucuronide, a previously described by-product of DON-3-GlcAc production by RLM, was identified in urine of DON-exposed mice and rats. The discovery of several novel DON-derived glucuronides in animal urine requires adaptation of the currently used methods for DON-biomarker analysis.

Dataset Information

Adsorption of Deoxynivalenol (DON) from Corn Steep Liquor (CSL) by the Microsphere Adsorbent SA/CMC Loaded with Calcium.

Publications

Adsorption of Deoxynivalenol (DON) from Corn Steep Liquor (CSL) by the Microsphere Adsorbent SA/CMC Loaded with Calcium.

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