Ontology highlight
ABSTRACT:
SUBMITTER: Towers CG
PROVIDER: S-EPMC7233142 | biostudies-literature | 2019 Sep
REPOSITORIES: biostudies-literature
Towers Christina G CG Fitzwalter Brent E BE Regan Daniel D Goodspeed Andrew A Morgan Michael J MJ Liu Chang-Wei CW Gustafson Daniel L DL Thorburn Andrew A
Developmental cell 20190801 6
While autophagy is thought to be an essential process in some cancer cells, it is unknown if or how such cancer cells can circumvent autophagy inhibition. To address this, we developed a CRISPR/Cas9 assay with dynamic live-cell imaging to measure acute effects of knockout (KO) of autophagy genes compared to known essential and non-essential genes. In some cancer cells, autophagy is as essential for cancer cell growth as mRNA transcription or translation or DNA replication. However, even these hi ...[more]