Gene-edited human stem cell-derived ? cells from a patient with monogenic diabetes reverse preexisting diabetes in mice.

ABSTRACT: Differentiation of insulin-producing pancreatic ? cells from induced pluripotent stem cells (iPSCs) derived from patients with diabetes promises to provide autologous cells for diabetes cell replacement therapy. However, current approaches produce patient iPSC-derived ? (SC-?) cells with poor function in vitro and in vivo. Here, we used CRISPR-Cas9 to correct a diabetes-causing pathogenic variant in Wolfram syndrome 1 (WFS1) in iPSCs derived from a patient with Wolfram syndrome (WS). After differentiation to ? cells with our recent six-stage differentiation strategy, corrected WS SC-? cells performed robust dynamic insulin secretion in vitro in response to glucose and reversed preexisting streptozocin-induced diabetes after transplantation into mice. Single-cell transcriptomics showed that corrected SC-? cells displayed increased insulin and decreased expression of genes associated with endoplasmic reticulum stress. CRISPR-Cas9 correction of a diabetes-inducing gene variant thus allows for robust differentiation of autologous SC-? cells that can reverse severe diabetes in an animal model.

SUBMITTER: Maxwell KG 

PROVIDER: S-EPMC7233417 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature