Ontology highlight
ABSTRACT:
SUBMITTER: Medaglia C
PROVIDER: S-EPMC7234837 | biostudies-literature | 2017 Dec
REPOSITORIES: biostudies-literature
Science (New York, N.Y.) 20171207 6370
Cellular functions are strongly dependent on surrounding cells and environmental factors. Current technologies are limited in their ability to characterize the spatial location and gene programs of cells in poorly structured and dynamic niches. We developed a method, NICHE-seq, that combines photoactivatable fluorescent reporters, two-photon microscopy, and single-cell RNA sequencing (scRNA-seq) to infer the cellular and molecular composition of niches. We applied NICHE-seq to examine the high-o ...[more]