Dataset Information

Estimation of dietary ¹⁴C dose coefficient using ¹³C-labelled compound administration analysis.

ABSTRACT: Carbon-14 released from nuclear facilities has been assessed to contribute significantly to the radiation dose that people are exposed to through the food chain. However, the current dose coefficient for members of public, which is the ratio of the 50-year committed effective dose to ingested 1?Bq ¹⁴C, recommended by the International Commission on Radiological Protection (ICRP) is not based on experimental human metabolic data for ¹⁴C in nutrients and diet. Therefore, to validate the coefficient, we administered ¹³C-labelled nutrients consisting of four amino acids, three fatty acids, and one monosaccharide to volunteers as substitutes for ¹⁴C labelled nutrients and measured the ¹³C concentration in various excreta samples. Although metabolic models were constructed from the excretion data, a significant fraction of administered ¹³C was not recovered from some nutrients. The dose coefficients of ¹⁴C in uniformly labelled Japanese diet, which were estimated under several assumptions about the unrecoverable fraction, varied from (6.2?±?0.9) × 10^-11 to (8.9?±?4.4) × 10^-10?Sv?Bq^-1 and were approximately comparable to the current value of 5.8 × 10^-10?Sv?Bq^-1 recommended by the ICRP. Further studies are necessary to elucidate the metabolism of ¹⁴C in various nutrients in the unrecoverable fraction.

SUBMITTER: Masuda T

PROVIDER: S-EPMC7235250 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature

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Publications

Estimation of dietary 14C dose coefficient using 13C-labelled compound administration analysis.

Masuda Tsuyoshi T Yoshioka Toshitada T Takahashi Tomoyuki T Takeda Hiroshi H Hatta Hideo H Matsushita Kensaku K Tako Yasuhiro Y Takaku Yuichi Y Hisamatsu Shun'ichi S

Scientific reports 20200518 1

Carbon-14 released from nuclear facilities has been assessed to contribute significantly to the radiation dose that people are exposed to through the food chain. However, the current dose coefficient for members of public, which is the ratio of the 50-year committed effective dose to ingested 1 Bq 14C, recommended by the International Commission on Radiological Protection (ICRP) is not based on experimental human metabolic data for 14C in nutrients and diet. Therefore, to v ...[more]

PMID: 32424291

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Project description:BackgroundStable isotopically labelled organisms have found wide application in life science research including plant physiology, plant stress and defense as well as metabolism related sciences. Therefore, the reproducible production of plant material enriched with stable isotopes such as 13C and 15N is of considerable interest. A high degree of enrichment (> 96 atom %) with a uniformly distributed isotope (global labelling) is accomplished by a continuous substrate supply during plant growth/cultivation. In the case of plants, 13C-labelling can be achieved by growth in 13CO2(g) atmosphere while global 15N-labelling needs 15N- containing salts in the watering/nutrient solution. Here, we present a method for the preparation of 13C and 15N-labelled plants by the use of closed growth chambers and hydroponic nutrient supply. The method is exemplified with durum wheat.ResultsIn total, 330 g of globally 13C- and 295 g of 15N-labelled Triticum durum wheat was produced during 87 cultivation days. For this, a total of 3.88 mol of 13CO2(g) and 58 mmol of 15N were consumed. The degree of enrichment was determined by LC-HRMS and ranged between 96 and 98 atom % for 13C and 95-99 atom % for 15N, respectively. Additionally, the isotopically labelled plant extracts were successfully used for metabolome-wide internal standardisation of native T.durum plants. Application of an isotope-assisted LC-HRMS workflow enabled the detection of 652 truly wheat-derived metabolites out of which 143 contain N.ConclusionA reproducible cultivation which makes use of climate chambers and hydroponics was successfully adapted to produce highly enriched, uniformly 13C- and 15N-labelled wheat. The obtained plant material is suitable to be used in all kinds of isotope-assisted research. The described technical equipment and protocol can easily be applied to other plants to produce 13C-enriched biological samples when the necessary specific adaptations e.g. temperature and light regime, as well as nutrient supply are considered. Additionally, the 15N-labelling method can also be carried out under regular glasshouse conditions without the need for customised atmosphere.

Dataset Information

Estimation of dietary ¹⁴C dose coefficient using ¹³C-labelled compound administration analysis.

Publications

Estimation of dietary <sup>14</sup>C dose coefficient using <sup>13</sup>C-labelled compound administration analysis.

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