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Etanercept Inhibits B Cell Differentiation by Regulating TNFRII/TRAF2/NF-?B Signaling Pathway in Rheumatoid Arthritis.

ABSTRACT: Objective:To explore the role of B cells in rheumatoid arthritis (RA) and the potential effects and mechanisms of etanercept on B cells. Methods:In RA patients, the levels of tumor necrosis factor-? (TNF-?) and B cell activating factor (BAFF) were detected by ELISA. The percentage of B cell subsets was measured by flow cytometry. Laboratory indicators (rheumatoid factor, C-reactive protein, erythrocyte sedimentation rate) and clinical indicators (disease activity score in 28 joints, health assessment questionnaire score, swollen joint counts, tender joint counts) were measured. The correlation between B cell subsets and laboratory indicators or clinical indicators was analyzed. In mice, B cells proliferation was detected by CCK-8 kit. The expression of TNFRII and the percentage of B cell subsets in spleen were detected by flow cytometry. The expressions of TRAF2, p38, P-p38, p65, P-p65 in B cells were detected by WB. Results:The percentage of CD19-CD27+CD138+ plasma B cells was positively correlated with ESR or RF. Etanercept could decrease the percentage of CD19+ total B cells, CD19+CD27+ memory B cells and CD19-CD27+CD138+ plasma B cells, reduce the levels of TNF-?, BAFF, relieve clinical and laboratory indicators in RA patients. In addition, etanercept could inhibit the proliferation of B cells, bate the differentiation of transitional B cells to mature B cells, down-regulate the expression of TNFRII, TRAF2, P-p38, P-p65 in B cells. Conclusion:B cells act a key role in the pathogenesis of RA. Etanercept inhibits B cells differentiation by down-regulating TNFRII/TRAF2/NF-?B signaling pathway.

SUBMITTER: Cai XY

PROVIDER: S-EPMC7235293 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Etanercept Inhibits B Cell Differentiation by Regulating TNFRII/TRAF2/NF-<i>κ</i>B Signaling Pathway in Rheumatoid Arthritis.

Cai Xiao-Yu XY Zhu Yue Y Wang Chen C Tang Xiao-Yu XY Han Le L Shu Jin-Ling JL Zhang Xian-Zheng XZ Liang Fa-Qin FQ Ge Jing-Ru JR Xu Li L Mei Dan D Zhang Ling-Ling LL Wei Wei W

Frontiers in pharmacology 20200512

<h4>Objective</h4>To explore the role of B cells in rheumatoid arthritis (RA) and the potential effects and mechanisms of etanercept on B cells.<h4>Methods</h4>In RA patients, the levels of tumor necrosis factor-α (TNF-α) and B cell activating factor (BAFF) were detected by ELISA. The percentage of B cell subsets was measured by flow cytometry. Laboratory indicators (rheumatoid factor, C-reactive protein, erythrocyte sedimentation rate) and clinical indicators (disease activity score in 28 joint ...[more]

PMID: 32477138

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Dataset Information

Etanercept Inhibits B Cell Differentiation by Regulating TNFRII/TRAF2/NF-?B Signaling Pathway in Rheumatoid Arthritis.

Publications

Etanercept Inhibits B Cell Differentiation by Regulating TNFRII/TRAF2/NF-<i>κ</i>B Signaling Pathway in Rheumatoid Arthritis.

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