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Endurance exercise training in pulmonary hypertension increases skeletal muscle electron transport chain supercomplex assembly.


ABSTRACT: Pulmonary hypertension is associated with pronounced exercise intolerance (decreased V???O2 max) that can significantly impact quality of life. The cause of exercise intolerance in pulmonary hypertension remains unclear. Mitochondrial supercomplexes are large respiratory assemblies of individual electron transport chain complexes which can promote more efficient respiration. In this study, we examined pulmonary hypertension and exercise-induced changes in skeletal muscle electron transport chain protein expression and supercomplex assembly. Pulmonary arterial hypertension was induced in rats with the Sugen/Hypoxia model (10% FiO2, three weeks). Pulmonary arterial hypertension and control rats were assigned to an exercise training protocol group or kept sedentary for one month. Cardiac function and V???O2 max were assessed at the beginning and end of exercise training. Red (Type 1-oxidative muscle) and white (Type 2-glycolytic muscle) gastrocnemius were assessed for changes in electron transport chain complex protein expression and supercomplex assembly via SDS- and Blue Native-PAGE. Results showed that pulmonary arterial hypertension caused a significant decrease in V???O2 max via treadmill testing that was improved with exercise (P?

SUBMITTER: McCullough DJ 

PROVIDER: S-EPMC7235683 | biostudies-literature | 2020 Apr-Jun

REPOSITORIES: biostudies-literature

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Endurance exercise training in pulmonary hypertension increases skeletal muscle electron transport chain supercomplex assembly.

McCullough Danielle J DJ   Kue Nouaying N   Mancini Thomas T   Vang Alexander A   Clements Richard T RT   Choudhary Gaurav G  

Pulmonary circulation 20200401 2


Pulmonary hypertension is associated with pronounced exercise intolerance (decreased V ċ O<sub>2</sub> max) that can significantly impact quality of life. The cause of exercise intolerance in pulmonary hypertension remains unclear. Mitochondrial supercomplexes are large respiratory assemblies of individual electron transport chain complexes which can promote more efficient respiration. In this study, we examined pulmonary hypertension and exercise-induced changes in skeletal muscle electron tran  ...[more]

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