Gene Alterations in Paired Supernatants and Precipitates from Malignant Pleural Effusions of Non-Squamous Non-Small Cell Lung Cancer.

ABSTRACT:

Objective

This study investigated the feasibility of using malignant pleural effusion (MPE) supernatant and paired cell blocks (precipitate) for gene profiling in patients with non-small cell lung cancer (NSCLC) using next-generation sequencing (NGS) technique.

Methods

Stage IV non-squamous NSCLC patients with MPE were eligible in this prospective study and recruited from Zhejiang Cancer Hospital between May 2014 and October 2015. MPE supernatant and paired precipitate sample gene alterations were determined with NGS containing 14 cancer-related genes. Progression free survival (PFS) was evaluated using Kaplan-Meier method and compared using log-rank test.

Results

A total of 102 patients were enrolled in the present study. All pleural effusions were confirmed as malig

SUBMITTER: Li J 

PROVIDER: S-EPMC7236047 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature