A Host-Specific Blocking Primer Combined with Optimal DNA Extraction Improves the Detection Capability of a Metabarcoding Protocol for Canine Vector-Borne Bacteria.
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ABSTRACT:
SUBMITTER: Huggins LG
PROVIDER: S-EPMC7238069 | biostudies-literature | 2020 Apr
REPOSITORIES: biostudies-literature
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A Host-Specific Blocking Primer Combined with Optimal DNA Extraction Improves the Detection Capability of a Metabarcoding Protocol for Canine Vector-Borne Bacteria.
Huggins Lucas G LG Koehler Anson V AV Schunack Bettina B Inpankaew Tawin T Traub Rebecca J RJ
Pathogens (Basel, Switzerland) 20200401 4
Bacterial canine vector-borne diseases are responsible for some of the most life-threatening conditions of dogs in the tropics and are typically poorly researched with some presenting a zoonotic risk to cohabiting people. Next-generation sequencing based methodologies have been demonstrated to accurately characterise a diverse range of vector-borne bacteria in dogs, whilst also proving to be more sensitive than conventional PCR techniques. We report two improvements to a previously developed met ...[more]
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