Dataset Information

Nrf2 Ablation Promotes Alzheimer's Disease-Like Pathology in APP/PS1 Transgenic Mice: The Role of Neuroinflammation and Oxidative Stress.

ABSTRACT:

Introduction

Alzheimer's disease (AD), the most common neurodegenerative disorder, is characterized by the accumulation of amyloid-? (A?) peptide and hyperphosphorylated tau protein. Accumulating evidence has revealed that the slow progressive deterioration of AD is associated with oxidative stress and chronic inflammation in the brain. Nuclear factor erythroid 2- (NF-E2-) related factor 2 (Nrf2), which acts through the Nrf2/ARE pathway, is a key regulator of the antioxidant and anti-inflammatory response. Although recent data show a link between Nrf2 and AD-related cognitive decline, the mechanism is still unknown. Thus, we explored how Nrf2 protects brain cells against the oxidative stress and inflammation of AD in a mouse model of AD (APP/PS1 transgenic (AT) mice) with genetic removal of Nrf2.

Methods

The spatial learning and memory abilities of 12-month-old transgenic mice were evaluated using a Morris water maze test. Hippocampal levels of Nrf2, A?, and p-tauS404 and of astrocytes and microglia were determined by immunostaining. Inflammatory cytokines were determined by ELISA and quantitative real-time polymerase chain reaction (qRT-PCR). Oxidative stress was measured by 8-hydroxydeoxyguanosine immunohistochemistry, and the antioxidant response was determined by qRT-PCR.

Results

The spatial learning and memory abilities of AT mice were impaired after Nrf2 deletion. A? and p-tauS404 accumulation was increased in the hippocampus of AT/Nrf2-KO mice. Astroglial and microglial activation was exacerbated, followed by upregulation of the proinflammatory cytokines IL-1?, IL-6, and TNF-?.

Conclusion

Our present results show that Nrf2 deficiency aggravates AD-like pathology in AT mice. This phenotype was associated with increased levels of oxidative and proinflammatory markers, which suggests that the Nrf2 pathway may be a promising therapeutic target for AD.

SUBMITTER: Ren P

PROVIDER: S-EPMC7238335 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Publications

Nrf2 Ablation Promotes Alzheimer's Disease-Like Pathology in APP/PS1 Transgenic Mice: The Role of Neuroinflammation and Oxidative Stress.

Ren Peng P Chen Jingwei J Li Bingxuan B Zhang Mengzhou M Yang Bei B Guo Xiangshen X Chen Ziyuan Z Cheng Hao H Wang Pengfei P Wang Shuaibo S Wang Ning N Zhang Guohua G Wu Xu X Ma Dan D Guan Dawei D Zhao Rui R

Oxidative medicine and cellular longevity 20200511

<h4>Introduction</h4>Alzheimer's disease (AD), the most common neurodegenerative disorder, is characterized by the accumulation of amyloid-<i>β</i> (A<i>β</i>) peptide and hyperphosphorylated tau protein. Accumulating evidence has revealed that the slow progressive deterioration of AD is associated with oxidative stress and chronic inflammation in the brain. Nuclear factor erythroid 2- (NF-E2-) related factor 2 (Nrf2), which acts through the Nrf2/ARE pathway, is a key regulator of the antioxidan ...[more]

PMID: 32454936

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Dataset Information

Nrf2 Ablation Promotes Alzheimer's Disease-Like Pathology in APP/PS1 Transgenic Mice: The Role of Neuroinflammation and Oxidative Stress.

Introduction

Methods

Results

Conclusion

Publications

Nrf2 Ablation Promotes Alzheimer's Disease-Like Pathology in APP/PS1 Transgenic Mice: The Role of Neuroinflammation and Oxidative Stress.

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