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Biological Evaluation of Newly Synthesized Biaryl Guanidine Derivatives to Arrest ?-Secretase Enzymatic Activity Involved in Alzheimer's Disease.


ABSTRACT: Proteases BACE1 (?-secretases) enzymes have been recognized as a promising target associated with Alzheimer's disease (AD). This study was carried out on the principles of molecular docking, chemical synthesis, and enzymatic inhibition of BACE1 enzymes via biaryl guanidine-based ligands. Based on virtual screening, thirteen different compounds were synthesized and subsequently evaluated via in vitro and in vivo studies. Among them, 1,3-bis(5,6-difluoropyridin-3-yl)guanidine (compound (9)) was found the most potent (IC50 = 97 ± 0.91?nM) and active to arrest (99%) ?-secretase enzymes (FRET assay). Furthermore, it was found to improve the novel object recognition test and Morris water maze test significantly (p < 0.05). Improved pharmacokinetic parameters, viz., Log?P o/w (1.76), Log?S (-2.73), and better penetration to the brain (BBB permeation) with zero Lipinski violation, made it possible to hit the BACE1 as a potential therapeutic source for AD.

SUBMITTER: Ali S 

PROVIDER: S-EPMC7238388 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Biological Evaluation of Newly Synthesized Biaryl Guanidine Derivatives to Arrest <i>β</i>-Secretase Enzymatic Activity Involved in Alzheimer's Disease.

Ali Sayyad S   Asad Muhammad Hassham Hassan Bin MHHB   Khan Fahad F   Murtaza Ghulam G   Rizvanov Albert A AA   Iqbal Jamshed J   Babak Borhan B   Hussain Izhar I  

BioMed research international 20200511


Proteases BACE1 (<i>β</i>-secretases) enzymes have been recognized as a promising target associated with Alzheimer's disease (AD). This study was carried out on the principles of molecular docking, chemical synthesis, and enzymatic inhibition of BACE1 enzymes via biaryl guanidine-based ligands. Based on virtual screening, thirteen different compounds were synthesized and subsequently evaluated via <i>in vitro</i> and <i>in vivo</i> studies. Among them, 1,3-bis(5,6-difluoropyridin-3-yl)guanidine  ...[more]

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