Rapid Detection of 2019 Novel Coronavirus SARS-CoV-2 Using a CRISPR-based DETECTR Lateral Flow Assay.
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ABSTRACT:
SUBMITTER: Broughton JP
PROVIDER: S-EPMC7239074 | biostudies-literature | 2020 Mar
REPOSITORIES: biostudies-literature
Publications
Rapid Detection of 2019 Novel Coronavirus SARS-CoV-2 Using a CRISPR-based DETECTR Lateral Flow Assay.
Broughton James P JP Deng Xianding X Yu Guixia G Fasching Clare L CL Singh Jasmeet J Streithorst Jessica J Granados Andrea A Sotomayor-Gonzalez Alicia A Zorn Kelsey K Gopez Allan A Hsu Elaine E Gu Wei W Miller Steve S Pan Chao-Yang CY Guevara Hugo H Wadford Debra A DA Chen Janice S JS Chiu Charles Y CY
medRxiv : the preprint server for health sciences 20200327
An outbreak of novel betacoronavirus, SARS-CoV-2 (formerly named 2019-nCoV), began in Wuhan, China in December 2019 and the COVID-19 disease associated with infection has since spread rapidly to multiple countries. Here we report the development of SARS-CoV-2 DETECTR, a rapid (~30 min), low-cost, and accurate CRISPR-Cas12 based lateral flow assay for detection of SARS-CoV-2 from respiratory swab RNA extracts. We validated this method using contrived reference samples and clinical samples from in ...[more]
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