Re-evaluation of Streptococcus pneumoniae carriage in Portuguese elderly by qPCR increases carriage estimates and unveils an expanded pool of serotypes.
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ABSTRACT:
SUBMITTER: Almeida ST
PROVIDER: S-EPMC7239868 | biostudies-literature | 2020 May
REPOSITORIES: biostudies-literature
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Re-evaluation of Streptococcus pneumoniae carriage in Portuguese elderly by qPCR increases carriage estimates and unveils an expanded pool of serotypes.
Almeida Sónia T ST Pedro Tânia T Paulo A Cristina AC de Lencastre Hermínia H Sá-Leão Raquel R
Scientific reports 20200520 1
Streptococcus pneumoniae (pneumococcus) is a leading cause of infections worldwide. Disease is preceded by asymptomatic colonization of the upper respiratory tract. Classical culture-based methods (CCBM) suggest that colonization in the elderly is <5%. Recently, use of qPCR has challenged these observations. We estimated pneumococcal carriage prevalence and serotypes among Portuguese elderly using qPCR and compared results with those obtained by CCBM. Nasopharyngeal and oropharyngeal paired samp ...[more]
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