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Ion torrent-based nasopharyngeal swab metatranscriptomics in COVID-19.

ABSTRACT: Herein, we describe the detection of a SARS-CoV-2 genome through metatranscriptome next-generation sequencing directly from the nasopharyngeal swab of a suspected case of local transmission of Covid-19, in Brazil. Depletion of human ribosomal RNA and use of an optimized in-house developed bioinformatics strategy contributed to successful detection of the virus.

SUBMITTER: Campos GS

PROVIDER: S-EPMC7240266 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature

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Ion torrent-based nasopharyngeal swab metatranscriptomics in COVID-19.

Campos Gubio S GS Sardi Silvia I SI Falcao Melissa B MB Belitardo Emilia M M A EMMA Rocha Danilo J P G DJPG Rolo Carolina A CA Menezes Aline D AD Pinheiro Carina S CS Carvalho Rejane H RH Almeida João P P JPP Aguiar Eric R G R ERGR Pacheco Luis G C LGC

Journal of virological methods 20200521

Herein, we describe the detection of a SARS-CoV-2 genome through metatranscriptome next-generation sequencing directly from the nasopharyngeal swab of a suspected case of local transmission of Covid-19, in Brazil. Depletion of human ribosomal RNA and use of an optimized in-house developed bioinformatics strategy contributed to successful detection of the virus. ...[more]

PMID: 32445875

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