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In Vitro and In Vivo Comparison Study of Electrospun PLA and PLA/PVA/SA Fiber Membranes for Wound Healing.

ABSTRACT: Wound dressings can accelerate wound healing. The degradable polymer poly(lactic acid) (PLA) shows good mechanical properties and biocompatibility. Sodium alginate (SA) holds good biocompatibility, hemostasis, and high hygroscopicity. Poly(vinyl alcohol) (PVA) has good spinnability as a pharmaceutical excipient. Herein, we carried out a comparison study of electrospun PLA and PLA/PVA/SA fiber membranes for wound healing in vitro and in vivo. In this study, PLA and PLA/PVA/SA nanofiber membranes were fabricated through electrospinning to produce a highly porous and large specific surface area that could promote wound healing. In vitro experiments showed that PLA and PLA/PVA/SA nanofiber membranes could all provide good support for the growth of rat fibroblasts (L929). Moreover, rat fibroblasts displayed slightly better adhesion and proliferation on PLA/PVA/SA than on the PLA fiber membranes. The in vivo potentiality of the PLA and PLA/PVA/SA fiber membranes was assessed in rat models of skin defects in which the PLA and PLA/PVA/SA fiber membranes significantly improved wound healing compared to commercially available gauzes. No significant differences in wound healing were observed between PLA and PLA/PVA/SA fiber membranes in our study. Furthermore, Masson staining and PCR displayed the PLA fiber membrane promoted protein deposition compared to the PLA/PVA/SA fiber membrane. In addition, IHC suggested that PLA/PVA/SA dressing reduced the inflammatory response during early wound healing compared to the PLA fiber membrane. These findings highlight the utility of fiber membranes as novel wound-healing dressings.

SUBMITTER: Bi H

PROVIDER: S-EPMC7240532 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature

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In Vitro and In Vivo Comparison Study of Electrospun PLA and PLA/PVA/SA Fiber Membranes for Wound Healing.

Bi Hongyan H Feng Tianyi T Li Binbin B Han Yingchao Y

Polymers 20200406 4

Wound dressings can accelerate wound healing. The degradable polymer poly(lactic acid) (PLA) shows good mechanical properties and biocompatibility. Sodium alginate (SA) holds good biocompatibility, hemostasis, and high hygroscopicity. Poly(vinyl alcohol) (PVA) has good spinnability as a pharmaceutical excipient. Herein, we carried out a comparison study of electrospun PLA and PLA/PVA/SA fiber membranes for wound healing in vitro and in vivo. In this study, PLA and PLA/PVA/SA nanofiber membranes ...[more]

PMID: 32268612

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Project description:In this study, we aim to develop organic–inorganic hybrid nanofibers containing high moisture retention and good mechanical performance as an antimicrobial dressing platform. The main theme of this work focuses on several technical tasks including (a) the electrospinning process (ESP) to produce organic polyvinyl alcohol/sodium alginate (PVA/SA) nanofibers with an excellent diameter uniformity and fibrous orientation, (b) the fabrication of inorganic nanoparticles (NPs) as graphene oxide (GO) and ZnO NPs to be added to PVA/SA nanofibers for enhancement of the mechanical properties and an antibacterial function to Staphylococcus aureus (S. aureus), and then (c) the crosslinking process for PVA/SA/GO/ZnO hybrid nanofibers in glutaraldehyde (GA) vapor atmosphere to improve the hydrophilicity and moisture absorption of specimens. Our results clearly indicate that the uniformity nanofiber with 7 wt% PVA and 2 wt% SA condition demonstrates 199 ± 22 nm in diameter using an electrospinning precursor solution of 355 cP in viscosity by the ESP process. Moreover, the mechanical strength of nanofibers was enhanced by 17% after the handling of a 0.5 wt% GO nanoparticles addition. Significantly, the morphology and size of ZnO NPs can be affected by NaOH concentration, where 1 M NaOH was used in the synthesis of 23 nm ZnO NPs corresponding to effective inhibition of S. aureus strains. The PVA/SA/GO/ZnO mixture successfully performed an antibacterial ability with an 8 mm inhibition zone in S. aureus strains. Furthermore, the GA vapor as a crosslinking agent acting on PVA/SA/GO/ZnO nanofiber provided both swelling behavior and structural stability performance. The swelling ratio increased up to 1.406%, and the mechanical strength was 1.87 MPa after 48 h of GA vapor treatment. Finally, we successfully synthesized the hybrid nanofibers of GA-treated PVA/SA/GO/ZnO accompanied with high moisturizing, biocompatibility, and great mechanical properties, which will be a novel multi-functional candidate for wound dressing composites for patients receiving surgical operations and first aid treatments.

Dataset Information

In Vitro and In Vivo Comparison Study of Electrospun PLA and PLA/PVA/SA Fiber Membranes for Wound Healing.

Publications

In Vitro and In Vivo Comparison Study of Electrospun PLA and PLA/PVA/SA Fiber Membranes for Wound Healing.

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