Dataset Information

A Multivariate Metabolomics Method for Estimating Platelet Mitochondrial Oxygen Consumption Rates in Patients with Sepsis.

ABSTRACT: BACKGROUND:Sepsis-induced alterations in mitochondrial function contribute to organ dysfunction and mortality. Measuring mitochondrial function in vital organs is neither feasible nor practical, highlighting the need for non-invasive approaches. Mitochondrial function may be reflected in the concentrations of metabolites found in platelets and whole blood (WB) samples. We proposed to use these as alternates to indirectly estimate platelet mitochondrial oxygen consumption rate (mOCR) in sepsis patients. METHODS:We determined the relationships between platelet mOCR and metabolites in both platelets and WB, as measured by quantitative 1H-NMR metabolomics. The associations were identified by building multiple linear regression models with stepwise forward-backward variable selection. We considered the models to be significant with an ANOVA test (p-value ? 0.05) and a positive predicted-R2. RESULTS:The differences in adjusted-R2 and ANOVA p-values (platelet adj-R2: 0.836 (0.0003), 0.711 (0.0004) vs. WB adj-R2: 0.428 (0.0079)) from the significant models indicate the platelet models were more associated with platelet mOCR. CONCLUSIONS:Our data suggest there are groups of metabolites in WB (leucine, acetylcarnitine) and platelets (creatine, ADP, glucose, taurine) that are associated with platelet mOCR. Thus, WB and platelet metabolites could be used to estimate platelet mOCR.

SUBMITTER: McCann MR

PROVIDER: S-EPMC7240966 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature

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A Multivariate Metabolomics Method for Estimating Platelet Mitochondrial Oxygen Consumption Rates in Patients with Sepsis.

McCann Marc R MR McHugh Cora E CE Kirby Maggie M Jennaro Theodore S TS Jones Alan E AE Stringer Kathleen A KA Puskarich Michael A MA

Metabolites 20200402 4

<h4>Background</h4>Sepsis-induced alterations in mitochondrial function contribute to organ dysfunction and mortality. Measuring mitochondrial function in vital organs is neither feasible nor practical, highlighting the need for non-invasive approaches. Mitochondrial function may be reflected in the concentrations of metabolites found in platelets and whole blood (WB) samples. We proposed to use these as alternates to indirectly estimate platelet mitochondrial oxygen consumption rate (mOCR) in s ...[more]

PMID: 32252461

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Project description:Monitoring aerobic exercise intensities of free-living physical activities is valuable for purposes such as education and research. The heart rate (HR) method, based on the linear relation between HR and oxygen uptake (VO2), is potentially valuable for this purpose. Three prerequisites are that the method is reproducible, and valid for the specific form of physical activity executed as well as under field conditions. The aim of this study is to evaluate reproducibility of the heart rate method in the laboratory. VO2 and HR measurements were made on two different occasions during three submaximal (model 1) plus a maximal exercise intensity (model 2) on a cycle ergometer in the laboratory. 19 habitual commuter cyclists (9 males and 10 females), aged 44 ± 3 years, were measured. The reproducibility of the estimated VO2, based on three levels of HR from commuting cycling and the regression equations from test and retest were analyzed. Differences between the two models were also studied. For both models, there were no significant differences between test and retest in the constituents of the regression equations (y-intercept, slope and r-value). Neither were there any systematic differences in estimated absolute levels of VO2 between test and retest. The relative differences between test and retest, based on estimations from three different levels of HR, were 0.99 ± 11.0 (n.s.), 2.67 ± 6.48 (n.s.) and 3.57 ± 6.24% (p<0.05) for model 1, and 1.09 ± 10.6, 1.75 ± 6.43 and 2.12 ± 5.92% (all n.s.) for model 2. However, some large individual differences were seen in both models. There were no significant differences between the two models in the slopes, intercepts or r-values of the regression equations or in the estimated levels of VO2. The heart rate method shows good reproducibility on the group level in estimating oxygen consumption from HR-VO2 relations in the laboratory, and based on three levels of HR which are representative for cycle commuting. However, on the individual level, some large variations were seen.

Dataset Information

A Multivariate Metabolomics Method for Estimating Platelet Mitochondrial Oxygen Consumption Rates in Patients with Sepsis.

Publications

A Multivariate Metabolomics Method for Estimating Platelet Mitochondrial Oxygen Consumption Rates in Patients with Sepsis.

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