PDGFR-? Promoter Forms a Vacancy G-Quadruplex that Can Be Filled in by dGMP: Solution Structure and Molecular Recognition of Guanine Metabolites and Drugs.

ABSTRACT: Aberrant expression of PDGFR-? is associated with a number of diseases. The G-quadruplexes (G4s) formed in PDGFR-? gene promoter are transcriptional modulators and amenable to small molecule targeting. The major G4 formed in the PDGFR-? gene promoter was previously shown to have a broken G-strand. Herein, we report that the PDGFR-? gene promoter sequence forms a vacancy G-quadruplex (vG4) which can be filled in and stabilized by physiologically relevant guanine metabolites, such as dGMP, GMP, and cGMP, as well as guanine-derivative drugs. We determined the NMR structure of the dGMP-fill-in PDGFR-? vG4 in K⁺ solution. This is the first structure of a guanine-metabolite-fill-in vG4 based on a human gene promoter sequence. Our structure and systematic analysis elucidate the contributions of Hoogsten hydrogen bonds, sugar, and phosphate moieties to the specific G-vacancy fill-in. Intriguingly, an equilibrium of 3'- and 5'-end vG4s is present in the PDGFR-? promoter sequence, and dGMP favors the 5'-end fill-in. Guanine metabolites and drugs were tested and showed a conserved selectivity for the 5'-vacancy, except for cGMP. cGMP binds both the 3'- and 5'-end vG4s and forms two fill-in G4s with similar population. Significantly, guanine metabolites are involved in many physiological and pathological processes in human cells; thus, our results provide a structural basis to understand their potential regulatory functions by interaction with promoter vG4s. Moreover, the NMR structure can guide rational design of ligands that target the PDGFR-? vG4.

SUBMITTER: Wang KB 

PROVIDER: S-EPMC7241120 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature