Colorimetric detection of Escherichia coli using engineered bacteriophage and an affinity reporter system.
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ABSTRACT:
SUBMITTER: Singh S
PROVIDER: S-EPMC7241434 | biostudies-literature | 2019 Nov
REPOSITORIES: biostudies-literature
Publications
Colorimetric detection of Escherichia coli using engineered bacteriophage and an affinity reporter system.
Singh Sangita S Hinkley Troy T Nugen Sam R SR Talbert Joey N JN
Analytical and bioanalytical chemistry 20190911 27
Reporter phage systems have emerged as a promising technology for the detection of bacteria in foods and water. However, the sensitivity of these assays is often limited by the concentration of the expressed reporter as well as matrix interferences associated with the sample. In this study, bacteriophage T7 was engineered to overexpress mutated alkaline phosphatase fused to a carbohydrate-binding module (ALP*-CBM) following infection of E. coli to enable colorimetric detection in a model system. ...[more]
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