Image-Based Marker-Free Screening of GABA_A Agonists, Antagonists, and Modulators.

ABSTRACT: The ionotropic GABA_A receptors represent the main target for different groups of widely used drugs having hypnotic and anxiolytic effects. So far, most approaches used to assess GABA activity involve invasive low -throughput electrophysiological techniques or rely on fluorescent dyes, preventing the ability to conduct noninvasive and thus nonperturbing screens. To address this limitation, we have developed an automated marker-free cell imaging method, based on digital holographic microscopy (DHM). This technology allows the automatically screening of compounds in multiple plates without having to label the cells or use special plates. This methodological approach was first validated by screening the GABA_A receptor expressed in HEK cells using a selection of active com

SUBMITTER: Rappaz B 

PROVIDER: S-EPMC7243081 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature