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Supramolecular Nanoscaffolds within Cytomimetic Protocells as Signal Localization Hubs.

ABSTRACT: The programmed construction of functional synthetic cells requires spatial control over arrays of biomolecules within the cytomimetic environment. The mimicry of the natural hierarchical assembly of biomolecules remains challenging due to the lack of an appropriate molecular toolbox. Herein, we report the implementation of DNA-decorated supramolecular assemblies as dynamic and responsive nanoscaffolds for the localization of arrays of DNA signal cargo within hierarchically assembled complex coacervate protocells. Protocells stabilized with a semipermeable membrane allow trafficking of single-stranded DNA between neighboring protocells. DNA duplex operations demonstrate the responsiveness of the nanoscaffolds to different input DNA strands via the reversible release of DNA cargo. Moreover, a second population of coacervate protocells with nanoscaffolds featuring a higher affinity for the DNA cargo enabled chemically programmed communication between both protocell populations. This combination of supramolecular structure and function paves the way for the next generation of protocells imbued with programmable, lifelike behaviors.

SUBMITTER: Magdalena Estirado E

PROVIDER: S-EPMC7243252 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature

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Supramolecular Nanoscaffolds within Cytomimetic Protocells as Signal Localization Hubs.

Magdalena Estirado Eva E Mason Alexander F AF Alemán García Miguel Ángel MÁ van Hest Jan C M JCM Brunsveld Luc L

Journal of the American Chemical Society 20200507 20

The programmed construction of functional synthetic cells requires spatial control over arrays of biomolecules within the cytomimetic environment. The mimicry of the natural hierarchical assembly of biomolecules remains challenging due to the lack of an appropriate molecular toolbox. Herein, we report the implementation of DNA-decorated supramolecular assemblies as dynamic and responsive nanoscaffolds for the localization of arrays of DNA signal cargo within hierarchically assembled complex coac ...[more]

PMID: 32356660

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Supramolecular Nanoscaffolds within Cytomimetic Protocells as Signal Localization Hubs.

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Supramolecular Nanoscaffolds within Cytomimetic Protocells as Signal Localization Hubs.

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