Relationship between Mecp2 and NF?b signaling during neural differentiation of P19 cells.

ABSTRACT: The pluripotent P19 embryo carcinoma cell line was studied to determine a signaling pathway regulating MeCP2 expression. P19 cells were induced to differentiate into neurons by RA and express ?-III tubulin at one day after induction and synaptophysin by 7 days. MeCP2 was first observed after ?-III tubulin expression was detected and continued to rise over the course of differentiation. Both Mecp2 e1 and e2 mRNA forms progressively increased in differentiating cells. MeCP2 expression was increased by tumor necrosis factor (TNF) in early differentiating cells, which was blocked by NF?B inhibitors. TNF did not increase MeCP2 expression in naïve cells. Moreover, TNF did not increase NF?B reporter gene activity in naïve cells even though increases were observed in early differentiating cells. The protein kinase C activator phorbol 12-myristate 13-acetate (PMA) increased MeCP2 expression in naïve P19 cells, which was also blocked by NF?B inhibitors. Interestingly, PMA increased NF?B reporter gene activity in naïve cells. Finally, PMA, but not TNF, induced I?B? degradation in naïve P19 cells. Taken together, our data indicates that MeCP2 expression is regulated in part by signaling pathways involving NF?B.

SUBMITTER: O'Driscoll C 

PROVIDER: S-EPMC7243855 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature