Variation within laminae: Semi-automated methods for quantifying leaf venation using phenoVein.

ABSTRACT:

Premise

Physiological processes may vary within leaf laminae; however, the accompanying heterogeneity in leaf venation is rarely investigated because its quantification can be time consuming. Here we introduce accelerated protocols using existing software to increase sample throughput and ask whether laminae venation varies among three crop types and four subspecies of Brassica rapa.

Methods

FAA (formaldehyde, glacial acetic acid, and ethanol)-fixed samples were stored in ethanol. Without performing any additional clearing or staining, we tested two methods of image acquisition at three locations along the proximal-distal axis of the laminae and estimated the patterns of venation using the program phenoVein. We developed and made available an R script to handle the ph

SUBMITTER: Newsome EL 

PROVIDER: S-EPMC7249269 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature