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MusiJ: an ImageJ plugin for video nanoscopy.


ABSTRACT: We present an open-source implementation of the fluctuation-based nanoscopy method MUSICAL for ImageJ. This implementation improves the algorithm's computational efficiency and takes advantage of multi-threading to provide orders of magnitude faster reconstructions than the original MATLAB implementation. In addition, the plugin is capable of generating super-resolution videos from large stacks of time-lapse images via an interleaved reconstruction, thus enabling easy-to-use multi-color super-resolution imaging of dynamic systems.

SUBMITTER: Acuna S 

PROVIDER: S-EPMC7249817 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature

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MusiJ: an ImageJ plugin for video nanoscopy.

Acuña Sebastian S   Ströhl Florian F   Opstad Ida S IS   Ahluwalia Balpreet S BS   Agarwal Krishna K  

Biomedical optics express 20200414 5


We present an open-source implementation of the fluctuation-based nanoscopy method MUSICAL for ImageJ. This implementation improves the algorithm's computational efficiency and takes advantage of multi-threading to provide orders of magnitude faster reconstructions than the original MATLAB implementation. In addition, the plugin is capable of generating super-resolution videos from large stacks of time-lapse images via an interleaved reconstruction, thus enabling easy-to-use multi-color super-re  ...[more]

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