Intratracheal aerosolization of viral vectors to newborn pig airways.
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ABSTRACT:
SUBMITTER: Cooney AL
PROVIDER: S-EPMC7252491 | biostudies-literature | 2020 May
REPOSITORIES: biostudies-literature
Publications
Intratracheal aerosolization of viral vectors to newborn pig airways.
Cooney Ashley L AL Sinn Patrick L PL
BioTechniques 20200306 5
Gene therapy for airway diseases requires efficient delivery of nucleic acids to the airways. In small animal models, gene delivery reagents are commonly delivered as a bolus dose. However, large animal models are often more relevant for the transition from preclinical studies to human trials. Aerosolizing viral vectors to the lungs of large animals can maximize anatomical distribution. Here, we describe a technique for aerosolization of viral vectors to the airways of newborn pigs. Briefly, a p ...[more]
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