Dataset Information

Evaluation of subgingival microbiota around single tooth implants.

ABSTRACT: Objectives:1. To provide information about the subgingival microbiota around single tooth implants.2. To assess the subgingival microbial flora around the teeth adjacent to single tooth implants.3. To clinically evaluate the gingival health surrounding the single-tooth implants. Methods:Patients undergoing the single-tooth implant replacements, were selected as subjects for the study. The natural teeth adjacent to implant sites were taken as control sites. Clinically each peri-implant gingival tissue health was evaluated. Subgingival plaque samples were removed with sterile curette and evaluated for microbial flora, by microscopic examinations. Bacterial cultures of samples studied. The similar procedure was followed for the control sites also. Finally the data collected were statistically analyzed and interpreted. Results:The subgingival microbiota around single tooth implants was cultured and studied. Enterobacter species, Klebsiella pneumonia, Pseudomonas aeruginosa and Streptococcus species were predominantly found. Klebsiella pneumonia and Pseudomonas aeruginosa were found more frequently around implant sites than control sites. Anaerobic Bacteroides species were found in only one case around the implant site. Conclusions:Prevention and control of bacterial infection in the peri-implant region are among the key factors in determining the long term success or failure of dental implant therapy. The thorough knowledge about the subgingival microbiota around the healthy and diseased peri-implant mucosa is needed to determine the overall outcome of implant therapy.

SUBMITTER: Tamrakar AK

PROVIDER: S-EPMC7254459 | biostudies-literature | 2020 Apr-Jun

REPOSITORIES: biostudies-literature

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Evaluation of subgingival microbiota around single tooth implants.

Tamrakar Amit Kumar AK Murali G G Singh Shyam S Shakila R R

Journal of oral biology and craniofacial research 20200415 2

<h4>Objectives</h4>1. To provide information about the subgingival microbiota around single tooth implants.2. To assess the subgingival microbial flora around the teeth adjacent to single tooth implants.3. To clinically evaluate the gingival health surrounding the single-tooth implants.<h4>Methods</h4>Patients undergoing the single-tooth implant replacements, were selected as subjects for the study. The natural teeth adjacent to implant sites were taken as control sites. Clinically each peri-imp ...[more]

PMID: 32489818

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Project description:Analysis of gingival crevicular fluid (GCF) samples may give information of the identity of unattached (planktonic) subgingival bacteria, the 35 forefront candidates for systemic dispersal via ulcerated periodontal pocket epithelium. Our study represents the first one targeting the identity of bacteria in gingival crevicular fluid. Methodology/Principal findings: We determined bacterial species diversity in GCF samples of a group of periodontitis patients and delineated contributing bacterial and host-associated factors. Subgingival paper point (PP) samples from the same sites were taken for comparison. After DNA extraction, 16S rRNA genes were PCR amplified and DNA-DNA hybridization was performed using a microarray for over 300 bacterial species or groups. Altogether 133 species from 41 genera and 8 phyla 45 were detected with 9 to 62 and 18 to 64 species in GCF and PP samples, respectively, 46 per patient. Projection to latent structures by means of partial least squares (PLS) was applied to the multivariate data analysis. PLS regression analysis showed that species of genera including Campylobacter, Selenomonas, Porphyromonas, Catonella, Tannerella, Dialister, Peptostreptococcus, Streptococcus and Eubacterium had significant positive correlations and the number of teeth with low-grade attachment loss a significant negative correlation to species diversity in GCF samples. OPLS/O2PLS discriminant analysis revealed significant positive correlations to GCF sample group membership for species of genera Campylobacter, Leptotrichia, Prevotella, Dialister, Tannerella, Haemophilus, Fusobacterium, Eubacterium, and Actinomyces. Conclusions/Significance: Among a variety of detected species those traditionally classified as Gram-negative anaerobes growing in mature subgingival biofilms were the main predictors for species diversity in GCF samples as well as responsible for distinguishing GCF samples from PP samples. GCF bacteria may provide new prospects for studying dynamic properties of subgingival biofilms.

Dataset Information

Evaluation of subgingival microbiota around single tooth implants.

Publications

Evaluation of subgingival microbiota around single tooth implants.

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