Curvature-sensitive trans-assembly of human Atg8-family proteins in autophagy-related membrane tethering.

ABSTRACT: In macroautophagy, de novo formation of the double membrane-bound organelles, termed autophagosomes, is essential for engulfing and sequestering the cytoplasmic contents to be degraded in the lytic compartments such as vacuoles and lysosomes. Atg8-family proteins have been known to be responsible for autophagosome formation via membrane tethering and fusion events of precursor membrane structures. Nevertheless, how Atg8 proteins act directly upon autophagosome formation still remains enigmatic. Here, to further gain molecular insights into Atg8-mediated autophagic membrane dynamics, we study the two representative human Atg8 orthologs, LC3B and GATE-16, by quantitatively evaluating their intrinsic potency to physically tether lipid membranes in a chemically defined reconstitution system us

SUBMITTER: Taniguchi S 

PROVIDER: S-EPMC7255505 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature