Dataset Information

Imaging Brain Metabolism Using Hyperpolarized ¹³C Magnetic Resonance Spectroscopy.

ABSTRACT: Aberrant metabolism is a key factor in many neurological disorders. The ability to measure such metabolic impairment could lead to improved detection of disease progression, and development and monitoring of new therapeutic approaches. Hyperpolarized ¹³C magnetic resonance spectroscopy (MRS) is a developing imaging technique that enables non-invasive measurement of enzymatic activity in real time in living organisms. Primarily applied in the fields of cancer and cardiac disease so far, this metabolic imaging method has recently been used to investigate neurological disorders. In this review, we summarize the preclinical research developments in this emerging field, and discuss future prospects for this exciting technology, which has the potential to change the clinical paradigm for patients with neurological disorders.

SUBMITTER: Le Page LM

PROVIDER: S-EPMC7255622 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature

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Imaging Brain Metabolism Using Hyperpolarized <sup>13</sup>C Magnetic Resonance Spectroscopy.

Le Page Lydia M LM Guglielmetti Caroline C Taglang Celine C Chaumeil Myriam M MM

Trends in neurosciences 20200408 5

Aberrant metabolism is a key factor in many neurological disorders. The ability to measure such metabolic impairment could lead to improved detection of disease progression, and development and monitoring of new therapeutic approaches. Hyperpolarized <sup>13</sup>C magnetic resonance spectroscopy (MRS) is a developing imaging technique that enables non-invasive measurement of enzymatic activity in real time in living organisms. Primarily applied in the fields of cancer and cardiac disease so far ...[more]

PMID: 32353337

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Project description:Lipopolysaccharide (LPS) is a commonly used agent for induction of neuroinflammation in preclinical studies. Upon injection, LPS causes activation of microglia and astrocytes, whose metabolism alters to favor glycolysis. Assessing in vivo neuroinflammation and its modulation following therapy remains challenging, and new noninvasive methods allowing for longitudinal monitoring would be highly valuable. Hyperpolarized (HP) 13 C magnetic resonance spectroscopy (MRS) is a promising technique for assessing in vivo metabolism. In addition to applications in oncology, the most commonly used probe of [1-13 C] pyruvate has shown potential in assessing neuroinflammation-linked metabolism in mouse models of multiple sclerosis and traumatic brain injury. Here, we aimed to investigate LPS-induced neuroinflammatory changes using HP [1-13 C] pyruvate and HP 13 C urea. 2D chemical shift imaging following simultaneous intravenous injection of HP [1-13 C] pyruvate and HP 13 C urea was performed at baseline (day 0) and at days 3 and 7 post-intracranial injection of LPS (n = 6) or saline (n = 5). Immunofluorescence (IF) analyses were performed for Iba1 (resting and activated microglia/macrophages), GFAP (resting and reactive astrocytes) and CD68 (activated microglia/macrophages). A significant increase in HP [1-13 C] lactate production was observed at days 3 and 7 following injection, in the injected (ipsilateral) side of the LPS-treated mouse brain, but not in either the contralateral side or saline-injected animals. HP 13 C lactate/pyruvate ratio, without and with normalization to urea, was also significantly increased in the ipsilateral LPS-injected brain at 7 days compared with baseline. IF analyses showed a significant increase in CD68 and GFAP staining at 3 days, followed by increased numbers of Iba1 and GFAP positive cells at 7 days post-LPS injection. In conclusion, we can detect LPS-induced changes in the mouse brain using HP 13 C MRS, in alignment with increased numbers of microglia/macrophages and astrocytes. This study demonstrates that HP 13 C spectroscopy has substantial potential for providing noninvasive information on neuroinflammation.

Dataset Information

Imaging Brain Metabolism Using Hyperpolarized ¹³C Magnetic Resonance Spectroscopy.

Publications

Imaging Brain Metabolism Using Hyperpolarized <sup>13</sup>C Magnetic Resonance Spectroscopy.

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Dataset Information

Imaging Brain Metabolism Using Hyperpolarized 13C Magnetic Resonance Spectroscopy.

Publications

Imaging Brain Metabolism Using Hyperpolarized <sup>13</sup>C Magnetic Resonance Spectroscopy.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Imaging Brain Metabolism Using Hyperpolarized ¹³C Magnetic Resonance Spectroscopy.