Comparison of Alere q whole blood viral load with DBS and plasma viral load in the classification of HIV virological failure.

ABSTRACT:

Background

In remote settings, timely plasma separation and transportation to testing laboratories is an impediment to the access of HIV viral load (VL) testing. Potential solutions are whole blood testing through point of care (POC) assays or dried blood spots (DBS).

Methods

We evaluated the performance of a prototype Alere q whole blood VL protocol and compared it against plasma (Abbott RealTime HIV-1) and DBS VL (Abbott RealTime HIV-1 DBS revised prototype protocol and Roche CAP/CTM HIV-1 v2.0 DBS free virus elution protocol). Virological failure (VF) was defined at >1000 copies/ml.

Results

Of 299 samples, Alere q correctly classified VF in 61% versus 87% by Abbott DBS and 76% by Roche FVE. Performance varied across plasma VL categories. Alere q showed 100% sensiti

SUBMITTER: Khan A 

PROVIDER: S-EPMC7259604 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature