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Emergence of a novel immune-evasion strategy from an ancestral protein fold in bacteriophage Mu.

ABSTRACT: The broad host range bacteriophage Mu employs a novel 'methylcarbamoyl' modification to protect its DNA from diverse restriction systems of its hosts. The DNA modification is catalyzed by a phage-encoded protein Mom, whose mechanism of action is a mystery. Here, we characterized the co-factor and metal-binding properties of Mom and provide a molecular mechanism to explain 'methylcarbamoyl'ation of DNA by Mom. Computational analyses revealed a conserved GNAT (GCN5-related N-acetyltransferase) fold in Mom. We demonstrate that Mom binds to acetyl CoA and identify the active site. We discovered that Mom is an iron-binding protein, with loss of Fe2+/3+-binding associated with loss of DNA modification activity. The importance of Fe2+/3+ is highlighted by the colocalization of Fe2+/3+ with acetyl CoA within the Mom active site. Puzzlingly, acid-base mechanisms employed by >309,000 GNAT members identified so far, fail to support methylcarbamoylation of adenine using acetyl CoA. In contrast, free-radical chemistry catalyzed by transition metals like Fe2+/3+ can explain the seemingly challenging reaction, accomplished by collaboration between acetyl CoA and Fe2+/3+. Thus, binding to Fe2+/3+, a small but unprecedented step in the evolution of Mom, allows a giant chemical leap from ordinary acetylation to a novel methylcarbamoylation function, while conserving the overall protein architecture.

SUBMITTER: Karambelkar S

PROVIDER: S-EPMC7261163 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature

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Emergence of a novel immune-evasion strategy from an ancestral protein fold in bacteriophage Mu.

Karambelkar Shweta S Udupa Shubha S Gowthami Vykuntham Naga VN Ramachandra Sharmila Giliyaru SG Swapna Ganduri G Nagaraja Valakunja V

Nucleic acids research 20200601 10

The broad host range bacteriophage Mu employs a novel 'methylcarbamoyl' modification to protect its DNA from diverse restriction systems of its hosts. The DNA modification is catalyzed by a phage-encoded protein Mom, whose mechanism of action is a mystery. Here, we characterized the co-factor and metal-binding properties of Mom and provide a molecular mechanism to explain 'methylcarbamoyl'ation of DNA by Mom. Computational analyses revealed a conserved GNAT (GCN5-related N-acetyltransferase) fol ...[more]

PMID: 32369169

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Dataset Information

Emergence of a novel immune-evasion strategy from an ancestral protein fold in bacteriophage Mu.

Publications

Emergence of a novel immune-evasion strategy from an ancestral protein fold in bacteriophage Mu.

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