Single-Cell Analysis for Glycogen Localization and Metabolism in Cultured Astrocytes.

ABSTRACT: Cerebral glycogen is principally localized in astrocytes rather than in neurons. Glycogen metabolism has been implicated in higher brain functions, including learning and memory, yet the distribution patterns of glycogen in different types of astrocytes have not been fully described. Here, we applied a method based on the incorporation of 2-NBDG, a D-glucose fluorescent derivative that can trace glycogen, to investigate glycogen's distribution in the brain. We identified two types of astrocytes, namely, 2-NBDG^I (glycogen-deficient) and 2-NBDG^II (glycogen-rich) cells. Whole-cell patch-clamp and fluorescence-activated cell sorting (FACS) were used to separate 2-NBDG^II astrocytes from 2-NBDG^I astrocytes. The expression levels of glycogen metabolic e

SUBMITTER: Zhu Y 

PROVIDER: S-EPMC7261284 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature